Rapid screening of bioactive compounds from natural products by integrating 5-channel parallel chromatography coupled with on-line mass spectrometry and microplate based assays

Yufeng Zhang, Shun Xiao, Lijuan Sun, Zhiwei Ge, Fengkai Fang, Wen Zhang, Yi Wang, Yiyu Cheng

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

A high throughput method was developed for rapid screening and identification of bioactive compounds from traditional Chinese medicine, marine products and other natural products. The system, integrated with five-channel chromatographic separation and dual UV-MS detection, is compatible with in vitro 96-well microplate based bioassays. The stability and applicability of the proposed method was validated by testing radical scavenging capability of a mixture of seven known compounds (rutin, dihydroquercetin, salvianolic acid A, salvianolic acid B, glycyrrhizic acid, rubescensin A and tangeretin). Moreover, the proposed method was successfully applied to the crude extracts of traditional Chinese medicine and a marine sponge from which 12 bioactive compounds were screened and characterized based on their anti-oxidative or anti-tumor activities. In particular, two diterpenoid derivatives, agelasine B and (-)-agelasine D, were identified for the first time as anti-tumor compounds from the sponge Agelas mauritiana, showing a considerable activity toward MCF-7 cells (IC50 values of 7.84±0.65 and 10.48±0.84μM, respectively). Our findings suggested that the integrated system of 5-channel parallel chromatography coupled with on-line mass spectrometry and microplate based assays can be a versatile and high efficient approach for the discovery of active compounds from natural products.

Original languageEnglish (US)
Pages (from-to)49-56
Number of pages8
JournalAnalytica Chimica Acta
Volume777
DOIs
StatePublished - May 13 2013

Keywords

  • Natural products
  • Parallel chromatography
  • Rapid identification
  • Targeted screening
  • Traditional Chinese medicine

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