TY - JOUR
T1 - Rapid scanning structure-activity relationships in combinatorial data sets
T2 - Identification of activity switches
AU - Medina-Franco, José L.
AU - Edwards, Bruce S.
AU - Pinilla, Clemencia
AU - Appel, Jon R.
AU - Giulianotti, Marc A.
AU - Santos, Radleigh G.
AU - Yongye, Austin B.
AU - Sklar, Larry A.
AU - Houghten, Richard A.
PY - 2013/6/24
Y1 - 2013/6/24
N2 - We present a general approach to describe the structure-activity relationships (SAR) of combinatorial data sets with activity for two biological endpoints with emphasis on the rapid identification of substitutions that have a large impact on activity and selectivity. The approach uses dual-activity difference (DAD) maps that represent a visual and quantitative analysis of all pairwise comparisons of one, two, or more substitutions around a molecular template. Scanning the SAR of data sets using DAD maps allows the visual and quantitative identification of activity switches defined as specific substitutions that have an opposite effect on the activity of the compounds against two targets. The approach also rapidly identifies single- and double-target R-cliffs, i.e., compounds where a single or double substitution around the central scaffold dramatically modifies the activity for one or two targets, respectively. The approach introduced in this report can be applied to any analogue series with two biological activity endpoints. To illustrate the approach, we discuss the SAR of 106 pyrrolidine bis-diketopiperazines tested against two formylpeptide receptors obtained from positional scanning deconvolution methods of mixture-based libraries.
AB - We present a general approach to describe the structure-activity relationships (SAR) of combinatorial data sets with activity for two biological endpoints with emphasis on the rapid identification of substitutions that have a large impact on activity and selectivity. The approach uses dual-activity difference (DAD) maps that represent a visual and quantitative analysis of all pairwise comparisons of one, two, or more substitutions around a molecular template. Scanning the SAR of data sets using DAD maps allows the visual and quantitative identification of activity switches defined as specific substitutions that have an opposite effect on the activity of the compounds against two targets. The approach also rapidly identifies single- and double-target R-cliffs, i.e., compounds where a single or double substitution around the central scaffold dramatically modifies the activity for one or two targets, respectively. The approach introduced in this report can be applied to any analogue series with two biological activity endpoints. To illustrate the approach, we discuss the SAR of 106 pyrrolidine bis-diketopiperazines tested against two formylpeptide receptors obtained from positional scanning deconvolution methods of mixture-based libraries.
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U2 - 10.1021/ci400192y
DO - 10.1021/ci400192y
M3 - Article
C2 - 23705689
AN - SCOPUS:84879562497
SN - 1549-9596
VL - 53
SP - 1475
EP - 1485
JO - Journal of Chemical Information and Modeling
JF - Journal of Chemical Information and Modeling
IS - 6
ER -