Rapid mini-prep isolation of high-quality plasmid DNA from Lactococcus and Lactobacillus spp.

D. J. O'Sullivan, T. R. Klaenhammer

Research output: Contribution to journalComment/debatepeer-review

359 Scopus citations

Abstract

A simple, rapid plasmid mini-prep procedure for lactococci and lactobacilli which gives high yields and can be performed on overnight broth cultures is presented. Large plasmids were isolated from both lactococci and lactobacilli, including a 70-kb plasmid from Lactobacillus acidophilus C7. The purity of the resulting plasmid DNA makes it suitable for subsequent molecular manipulations. The convenience of the technique makes this rapid mini-prep procedure suitable for routine plasmid isolation from lactic acid bacteria.

Original languageEnglish (US)
Pages (from-to)2730-2733
Number of pages4
JournalApplied and environmental microbiology
Volume59
Issue number8
DOIs
StatePublished - 1993

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