Rapid microtubule self-assembly kinetics

Melissa K. Gardner, Blake D. Charlebois, Imre M. Jánosi, Jonathon Howard, Alan J. Hunt, David J. Odde

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

Microtubule assembly is vital for many fundamental cellular processes. Current models for microtubule assembly kinetics assume that the subunit dissociation rate from a microtubule tip is independent of free subunit concentration. Total-Internal-Reflection-Fluorescence (TIRF) microscopy experiments and data from a laser tweezers assay that measures in vitro microtubule assembly with nanometer resolution, provides evidence that the subunit dissociation rate from a microtubule tip increases as the free subunit concentration increases. These data are consistent with a two-dimensional model for microtubule assembly, and are explained by a shift in microtubule tip structure from a relatively blunt shape at low free concentrations to relatively tapered at high free concentrations. We find that because both the association and the dissociation rates increase at higher free subunit concentrations, the kinetics of microtubule assembly are an order-of-magnitude higher than currently estimated in the literature.

Original languageEnglish (US)
Pages (from-to)582-592
Number of pages11
JournalCell
Volume146
Issue number4
DOIs
StatePublished - Aug 19 2011

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Microtubules
Self assembly
Kinetics
Fluorescence microscopy
Optical Tweezers
Assays
Association reactions
Fluorescence Microscopy
Lasers
Experiments

Cite this

Gardner, M. K., Charlebois, B. D., Jánosi, I. M., Howard, J., Hunt, A. J., & Odde, D. J. (2011). Rapid microtubule self-assembly kinetics. Cell, 146(4), 582-592. https://doi.org/10.1016/j.cell.2011.06.053

Rapid microtubule self-assembly kinetics. / Gardner, Melissa K.; Charlebois, Blake D.; Jánosi, Imre M.; Howard, Jonathon; Hunt, Alan J.; Odde, David J.

In: Cell, Vol. 146, No. 4, 19.08.2011, p. 582-592.

Research output: Contribution to journalArticle

Gardner, MK, Charlebois, BD, Jánosi, IM, Howard, J, Hunt, AJ & Odde, DJ 2011, 'Rapid microtubule self-assembly kinetics', Cell, vol. 146, no. 4, pp. 582-592. https://doi.org/10.1016/j.cell.2011.06.053
Gardner MK, Charlebois BD, Jánosi IM, Howard J, Hunt AJ, Odde DJ. Rapid microtubule self-assembly kinetics. Cell. 2011 Aug 19;146(4):582-592. https://doi.org/10.1016/j.cell.2011.06.053
Gardner, Melissa K. ; Charlebois, Blake D. ; Jánosi, Imre M. ; Howard, Jonathon ; Hunt, Alan J. ; Odde, David J. / Rapid microtubule self-assembly kinetics. In: Cell. 2011 ; Vol. 146, No. 4. pp. 582-592.
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