Rapid microassays for clindamycin and gentamicin when present together and the effect of pH and of each on the antibacterial activity of the other.

L. D. Sabath, I. Toftegaard

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Gentamicin was measured in the presence of clindamycin by using as the assay organism a strain of Staphylococcus aureus that was resistant to clindamycin; clindamycin was measured in the presence of gentamicin by using Clostridium perfringens as the assay organism. Both assays were in agar diffusion systems, were rapid (2 to 4 h), and had errors of less than 10%. Neither antibiotic antagonized or synergized with the other against a variety of organisms. The pK of clindamycin was 7.3, and the pK of gentamicin was 8.2. Gentamicin was 100 or more times as active in alkaline medium against both gram-positive and gram-negative organisms, but clindamycin showed a striking pH effect on activity only with gram-positive organisms.
Original languageUndefined/Unknown
Pages (from-to)54-59
Number of pages6
JournalAntimicrobial Agents and Chemotherapy
Volume6
Issue number1
StatePublished - 1974

Keywords

  • Anti-Bacterial Agents/pd [Pharmacology]
  • Clindamycin/pd [Pharmacology]
  • Clostridium perfringens/de [Drug Effects]
  • Drug Therapy, Combination/pd [Pharmacology]
  • Gentamicins/pd [Pharmacology]
  • Hydrogen-Ion Concentration
  • Microbial Sensitivity Tests

Cite this

@article{e31ec087240449159922248a52cda61b,
title = "Rapid microassays for clindamycin and gentamicin when present together and the effect of pH and of each on the antibacterial activity of the other.",
abstract = "Gentamicin was measured in the presence of clindamycin by using as the assay organism a strain of Staphylococcus aureus that was resistant to clindamycin; clindamycin was measured in the presence of gentamicin by using Clostridium perfringens as the assay organism. Both assays were in agar diffusion systems, were rapid (2 to 4 h), and had errors of less than 10{\%}. Neither antibiotic antagonized or synergized with the other against a variety of organisms. The pK of clindamycin was 7.3, and the pK of gentamicin was 8.2. Gentamicin was 100 or more times as active in alkaline medium against both gram-positive and gram-negative organisms, but clindamycin showed a striking pH effect on activity only with gram-positive organisms.",
keywords = "Anti-Bacterial Agents/pd [Pharmacology], Clindamycin/pd [Pharmacology], Clostridium perfringens/de [Drug Effects], Drug Therapy, Combination/pd [Pharmacology], Gentamicins/pd [Pharmacology], Hydrogen-Ion Concentration, Microbial Sensitivity Tests",
author = "Sabath, {L. D.} and I. Toftegaard",
year = "1974",
language = "Undefined/Unknown",
volume = "6",
pages = "54--59",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Rapid microassays for clindamycin and gentamicin when present together and the effect of pH and of each on the antibacterial activity of the other.

AU - Sabath, L. D.

AU - Toftegaard, I.

PY - 1974

Y1 - 1974

N2 - Gentamicin was measured in the presence of clindamycin by using as the assay organism a strain of Staphylococcus aureus that was resistant to clindamycin; clindamycin was measured in the presence of gentamicin by using Clostridium perfringens as the assay organism. Both assays were in agar diffusion systems, were rapid (2 to 4 h), and had errors of less than 10%. Neither antibiotic antagonized or synergized with the other against a variety of organisms. The pK of clindamycin was 7.3, and the pK of gentamicin was 8.2. Gentamicin was 100 or more times as active in alkaline medium against both gram-positive and gram-negative organisms, but clindamycin showed a striking pH effect on activity only with gram-positive organisms.

AB - Gentamicin was measured in the presence of clindamycin by using as the assay organism a strain of Staphylococcus aureus that was resistant to clindamycin; clindamycin was measured in the presence of gentamicin by using Clostridium perfringens as the assay organism. Both assays were in agar diffusion systems, were rapid (2 to 4 h), and had errors of less than 10%. Neither antibiotic antagonized or synergized with the other against a variety of organisms. The pK of clindamycin was 7.3, and the pK of gentamicin was 8.2. Gentamicin was 100 or more times as active in alkaline medium against both gram-positive and gram-negative organisms, but clindamycin showed a striking pH effect on activity only with gram-positive organisms.

KW - Anti-Bacterial Agents/pd [Pharmacology]

KW - Clindamycin/pd [Pharmacology]

KW - Clostridium perfringens/de [Drug Effects]

KW - Drug Therapy, Combination/pd [Pharmacology]

KW - Gentamicins/pd [Pharmacology]

KW - Hydrogen-Ion Concentration

KW - Microbial Sensitivity Tests

M3 - Article

VL - 6

SP - 54

EP - 59

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 1

ER -