Rapid identification and characterization of antioxidants from Ligularia fischeri

Xiang Lan Piao; Xiao Yuan Mi; Yan Ze Tian; Qian Wu; Hui Shan Piao; Zhikai Zeng; Ding Wang; Xiangshu Piao

doi:10.1007/s12272-009-2204-z

Rapid identification and characterization of antioxidants from Ligularia fischeri

Xiang Lan Piao, Xiao Yuan Mi, Yan Ze Tian, Qian Wu, Hui Shan Piao, Zhikai Zeng, Ding Wang, Xiangshu Piao

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The objectives of this study were to investigate the radical-scavenging activity of Ligularia fischeri on oxidative damage by the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and to rapidly identify the active components using the bioassay-linked fractionation method. The MeOH extract and fractions of CH₂Cl₂, BuOH, and H₂O from L. fischeri showed DPPH radical-scavenging effects in a dose-dependent manner (p < 0.01). In particular, the BuOH fraction had the most effective (p < 0.05) antioxidative capacity. The active constituents from the BuOH fraction of L. fischeri were rapidly isolated by bioassay-linked HPLC method and identified as hyperoside and 2″-acetylhyperoside with potent antioxidant effects against the DPPH radical, with IC₅₀ values of 1.31 and 7.09 μg/mL, respectively. They have not been reported from L. fischeri yet.

Original language	English (US)
Pages (from-to)	1689-1694
Number of pages	6
Journal	Archives of Pharmacal Research
Volume	32
Issue number	12
DOIs	https://doi.org/10.1007/s12272-009-2204-z
State	Published - Dec 2009
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported financially by the NSFC (No. 30973960; No. 30671522), a project of the State Ethnic Affairs Commission (No. 09ZY17), HYKJ (No. 2006BAD12B05-10) and Young Teacher Research Grant of Minzu University of China in 2008.

Keywords

2″-acetylhyperoside
Antioxidative activity
Bioassay-linked HPLC
Hyperoside
Ligularia fischeri

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10.1007/s12272-009-2204-z

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title = "Rapid identification and characterization of antioxidants from Ligularia fischeri",

abstract = "The objectives of this study were to investigate the radical-scavenging activity of Ligularia fischeri on oxidative damage by the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and to rapidly identify the active components using the bioassay-linked fractionation method. The MeOH extract and fractions of CH2Cl2, BuOH, and H2O from L. fischeri showed DPPH radical-scavenging effects in a dose-dependent manner (p < 0.01). In particular, the BuOH fraction had the most effective (p < 0.05) antioxidative capacity. The active constituents from the BuOH fraction of L. fischeri were rapidly isolated by bioassay-linked HPLC method and identified as hyperoside and 2″-acetylhyperoside with potent antioxidant effects against the DPPH radical, with IC50 values of 1.31 and 7.09 μg/mL, respectively. They have not been reported from L. fischeri yet.",

keywords = "2″-acetylhyperoside, Antioxidative activity, Bioassay-linked HPLC, Hyperoside, Ligularia fischeri",

author = "Piao, {Xiang Lan} and Mi, {Xiao Yuan} and Tian, {Yan Ze} and Qian Wu and Piao, {Hui Shan} and Zhikai Zeng and Ding Wang and Xiangshu Piao",

note = "Funding Information: This work was supported financially by the NSFC (No. 30973960; No. 30671522), a project of the State Ethnic Affairs Commission (No. 09ZY17), HYKJ (No. 2006BAD12B05-10) and Young Teacher Research Grant of Minzu University of China in 2008.",

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AU - Piao, Xiang Lan

AU - Mi, Xiao Yuan

AU - Tian, Yan Ze

AU - Wu, Qian

AU - Piao, Hui Shan

AU - Zeng, Zhikai

AU - Wang, Ding

AU - Piao, Xiangshu

N1 - Funding Information: This work was supported financially by the NSFC (No. 30973960; No. 30671522), a project of the State Ethnic Affairs Commission (No. 09ZY17), HYKJ (No. 2006BAD12B05-10) and Young Teacher Research Grant of Minzu University of China in 2008.

PY - 2009/12

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N2 - The objectives of this study were to investigate the radical-scavenging activity of Ligularia fischeri on oxidative damage by the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and to rapidly identify the active components using the bioassay-linked fractionation method. The MeOH extract and fractions of CH2Cl2, BuOH, and H2O from L. fischeri showed DPPH radical-scavenging effects in a dose-dependent manner (p < 0.01). In particular, the BuOH fraction had the most effective (p < 0.05) antioxidative capacity. The active constituents from the BuOH fraction of L. fischeri were rapidly isolated by bioassay-linked HPLC method and identified as hyperoside and 2″-acetylhyperoside with potent antioxidant effects against the DPPH radical, with IC50 values of 1.31 and 7.09 μg/mL, respectively. They have not been reported from L. fischeri yet.

AB - The objectives of this study were to investigate the radical-scavenging activity of Ligularia fischeri on oxidative damage by the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and to rapidly identify the active components using the bioassay-linked fractionation method. The MeOH extract and fractions of CH2Cl2, BuOH, and H2O from L. fischeri showed DPPH radical-scavenging effects in a dose-dependent manner (p < 0.01). In particular, the BuOH fraction had the most effective (p < 0.05) antioxidative capacity. The active constituents from the BuOH fraction of L. fischeri were rapidly isolated by bioassay-linked HPLC method and identified as hyperoside and 2″-acetylhyperoside with potent antioxidant effects against the DPPH radical, with IC50 values of 1.31 and 7.09 μg/mL, respectively. They have not been reported from L. fischeri yet.

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Rapid identification and characterization of antioxidants from Ligularia fischeri

Abstract

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