@inbook{e364727863ad4411985032b0de38d032,
title = "Rapid genetic modification of mouse embryonic stem cells by inducible cassette exchange recombination",
abstract = "Embryonic stem cell (ESC) differentiation is a useful means by which to produce large quantities of cells in vitro representing early stages of embryonic development. A conditional gene expression system allows interrogation of factors at specific time points in the differentiation of ES cells to defined cell types. We have developed a method for rapidly generating conditional inducible murine ES cells by targeting genes into an Inducible Cassette Exchange (ICE) locus. The ICE locus encodes a doxycycline-inducible floxed Cre, which replaces itself with an incoming floxed gene of interest. The derivative cell lines, selected in G418, thus bear doxycycline-inducible transgenes. We provide detailed methods for performing ICE recombination and generating derivative doxycycline-inducible cell lines.",
keywords = "Cre, ESC, ESC targeting, Inducible system, Recombination",
author = "Michelina Iacovino and Roth, {Megan E.} and Michael Kyba",
year = "2014",
doi = "10.1007/978-1-62703-721-1_16",
language = "English (US)",
isbn = "9781627037204",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "339--351",
booktitle = "Gene Function Analysis",
}