Rapid determination of free tryptophan in plant samples by gas chromatography-selected ion monitoring mass spectrometry

Lech Michalczuk; Krystyna Bialek; Jerry D. Cohen

doi:10.1016/0021-9673(92)85021-K

Rapid determination of free tryptophan in plant samples by gas chromatography-selected ion monitoring mass spectrometry

Lech Michalczuk, Krystyna Bialek, Jerry D. Cohen

Horticultural Science

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

An isotope dilution assay for plant tryptophan is described. The method consists of solid-phate extraction techniques, the one-step formation of the N-acetyl methyl ester derivative, followed by purification by C₁₈ high-performance liquid chromatography and analysis by gas chromatography-selected ion monitoring mass spectrometry. The method can be used effectively to measure free tryptophan in plant samples as small as 10 mg fresh weight.

Original language	English (US)
Pages (from-to)	294-298
Number of pages	5
Journal	Journal of Chromatography A
Volume	596
Issue number	2
DOIs	https://doi.org/10.1016/0021-9673(92)85021-K
State	Published - Apr 10 1992

Bibliographical note

Funding Information:
This work was supported by grants from the US-Israel Binational Agricultural Research and Development Fund (US-1362-87), USDA-CRGO Plant Growth and Development (89-37261-4734)a nd by funds from the USDA Agricultural Research Service.

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10.1016/0021-9673(92)85021-K

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title = "Rapid determination of free tryptophan in plant samples by gas chromatography-selected ion monitoring mass spectrometry",

abstract = "An isotope dilution assay for plant tryptophan is described. The method consists of solid-phate extraction techniques, the one-step formation of the N-acetyl methyl ester derivative, followed by purification by C18 high-performance liquid chromatography and analysis by gas chromatography-selected ion monitoring mass spectrometry. The method can be used effectively to measure free tryptophan in plant samples as small as 10 mg fresh weight.",

author = "Lech Michalczuk and Krystyna Bialek and Cohen, {Jerry D.}",

note = "Funding Information: This work was supported by grants from the US-Israel Binational Agricultural Research and Development Fund (US-1362-87), USDA-CRGO Plant Growth and Development (89-37261-4734)a nd by funds from the USDA Agricultural Research Service.",

year = "1992",

month = apr,

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doi = "10.1016/0021-9673(92)85021-K",

language = "English (US)",

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pages = "294--298",

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T1 - Rapid determination of free tryptophan in plant samples by gas chromatography-selected ion monitoring mass spectrometry

AU - Michalczuk, Lech

AU - Bialek, Krystyna

AU - Cohen, Jerry D.

N1 - Funding Information: This work was supported by grants from the US-Israel Binational Agricultural Research and Development Fund (US-1362-87), USDA-CRGO Plant Growth and Development (89-37261-4734)a nd by funds from the USDA Agricultural Research Service.

PY - 1992/4/10

Y1 - 1992/4/10

N2 - An isotope dilution assay for plant tryptophan is described. The method consists of solid-phate extraction techniques, the one-step formation of the N-acetyl methyl ester derivative, followed by purification by C18 high-performance liquid chromatography and analysis by gas chromatography-selected ion monitoring mass spectrometry. The method can be used effectively to measure free tryptophan in plant samples as small as 10 mg fresh weight.

AB - An isotope dilution assay for plant tryptophan is described. The method consists of solid-phate extraction techniques, the one-step formation of the N-acetyl methyl ester derivative, followed by purification by C18 high-performance liquid chromatography and analysis by gas chromatography-selected ion monitoring mass spectrometry. The method can be used effectively to measure free tryptophan in plant samples as small as 10 mg fresh weight.

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ER -

Rapid determination of free tryptophan in plant samples by gas chromatography-selected ion monitoring mass spectrometry

Abstract

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