Rapid detection of the Fusarium oxysporum lineage containing the Canary Island date palm wilt pathogen

T. R. Plyler, G. W. Simone, D. Fernandez, H. C. Kistler

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Fusarium oxysporum f. sp. canariensis causes Fusarium wilt disease on the Canary Island date palm (Phoenix canariensis). To facilitate disease management, a polymerase chain reaction diagnostic method has been developed to rapidly detect the pathogen. A partial genomic library of F. oxysporum f. sp. canariensis isolate 95-913 was used to identify a DNA sequence diagnostic for a lineage containing all tested isolates of F. oxysporum f. sp. canariensis. Two oligonucleotide primers were designed and used to amplify a 567-bp fragment with E oxysporum f. sp. canariensis DNAs. DNA from 61 outgroup isolates did not amplify using these primers. Once the primers were shown to amplify a 0.567-kb fragment from DNA of all the F. oxysporum f. sp. canariensis isolates tested, a rapid DNA extraction procedure was developed that led to the correct identification of 98% of the tested F. oxysporum f. sp. canariensis isolates.

Original languageEnglish (US)
Pages (from-to)407-413
Number of pages7
JournalPhytopathology
Volume89
Issue number5
DOIs
StatePublished - May 1999

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