Rapid Detection of Ricin in Milk Using Immunomagnetic Separation Combined with Surface-Enhanced Raman Spectroscopy

Lili He, Bronwyn Deen, Tom Rodda, Ian Ronningen, Tim Blasius, Christy Haynes, Francisco Diez-Gonzalez, Theodore P. Labuza

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Ricin is a potential bioterrisiom agent. There is a critical need for a method that can rapidly and simply detect ricin and other bioterrisiom agents in complex food matrices such as milk. In this study, we demonstrated a rapid method that combined immunomagnetic separation (IMS) and surface-enhanced Raman spectroscopy (SERS) to detect ricin in whole milk. IMS was used to specifically capture the ricin out of the milk. Then, SERS was applied to analyze the IMS eluate mixed with silver dendrite nanosubstrates. This approach facilitated detection and quantification down to 4 μg/mL ricin in milk within 20 min, based on the results of principal component analysis and partial least squares analysis. The feasibility of using a portable Raman instrument shows great promise for on-site detection in a processing facility.

Original languageEnglish (US)
Pages (from-to)N49-N53
JournalJournal of food science
Volume76
Issue number5
DOIs
StatePublished - Jun 1 2011

Keywords

  • IMS
  • Milk
  • Portable Raman
  • Ricin
  • SERS

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