We describe a patient with severe and progressive encephalitis of unknown etiology. We performed rapid metagenomic sequencing from cerebrospinal fluid and identified Powassan virus, an emerging tick-borne flavivirus that has been increasingly detected in the United States.
Bibliographical noteFunding Information:
Financial support. This work was supported by the National Institutes of Health (KL2 TR001100 to A. P., T32 AI007061 to S. K., and T32 AG000222 to S. S. M.), a Massachusetts General Hospital Department of Neurology SPARK Award to S. S. M. and T. C., and a generous Broadnext10 gift from the Broad Institute.
Potential conflicts of interest. S. T. reports consultation on tick-borne disease diagnostics with Oxford Immunotec, Fuller Laboratories, and Meridian Biosciences. E. R. reports consultation with T2 Biosystems and TBS Technologies. M. F. reports a sponsored research grant from Biogen. All other authors declare no potential conflicts. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.
- Metagenomic sequencing
- Powassan virus