TY - JOUR
T1 - Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture
AU - Steuernagel, Burkhard
AU - Periyannan, Sambasivam K.
AU - Hernández-Pinzón, Inmaculada
AU - Witek, Kamil
AU - Rouse, Matthew N.
AU - Yu, Guotai
AU - Hatta, Asyraf
AU - Ayliffe, Mick
AU - Bariana, Harbans
AU - Jones, Jonathan D.G.
AU - Lagudah, Evans S.
AU - Wulff, Brande B.H.
N1 - Publisher Copyright:
© 2016 Nature America, Inc. All rights reserved.
PY - 2016/6/1
Y1 - 2016/6/1
N2 - Wild relatives of domesticated crop species harbor multiple, diverse, disease resistance (R) genes that could be used to engineer sustainable disease control. However, breeding R genes into crop lines often requires long breeding timelines of 5-15 years to break linkage between R genes and deleterious alleles (linkage drag). Further, when R genes are bred one at a time into crop lines, the protection that they confer is often overcome within a few seasons by pathogen evolution. If several cloned R genes were available, it would be possible to pyramid R genes in a crop, which might provide more durable resistance. We describe a three-step method (MutRenSeq)-that combines chemical mutagenesis with exome capture and sequencing for rapid R gene cloning. We applied MutRenSeq to clone stem rust resistance genes Sr22 and Sr45 from hexaploid bread wheat. MutRenSeq can be applied to other commercially relevant crops and their relatives, including, for example, pea, bean, barley, oat, rye, rice and maize.
AB - Wild relatives of domesticated crop species harbor multiple, diverse, disease resistance (R) genes that could be used to engineer sustainable disease control. However, breeding R genes into crop lines often requires long breeding timelines of 5-15 years to break linkage between R genes and deleterious alleles (linkage drag). Further, when R genes are bred one at a time into crop lines, the protection that they confer is often overcome within a few seasons by pathogen evolution. If several cloned R genes were available, it would be possible to pyramid R genes in a crop, which might provide more durable resistance. We describe a three-step method (MutRenSeq)-that combines chemical mutagenesis with exome capture and sequencing for rapid R gene cloning. We applied MutRenSeq to clone stem rust resistance genes Sr22 and Sr45 from hexaploid bread wheat. MutRenSeq can be applied to other commercially relevant crops and their relatives, including, for example, pea, bean, barley, oat, rye, rice and maize.
UR - http://www.scopus.com/inward/record.url?scp=84973657517&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84973657517&partnerID=8YFLogxK
U2 - 10.1038/nbt.3543
DO - 10.1038/nbt.3543
M3 - Article
C2 - 27111722
AN - SCOPUS:84973657517
SN - 1087-0156
VL - 34
SP - 652
EP - 655
JO - Nature biotechnology
JF - Nature biotechnology
IS - 6
ER -
