Rapid and specific detection of the O15:K52:H1 clonal group of Escherichia coli by gene-specific PCR

James R. Johnson, Krista Owens, Montse Sabate, Guillem Prats

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Primers specific for Escherichia coli O15:K52:H1 were devised based on a novel single-nucleotide polymorphism identified within the housekeeping gene fumC, i.e., G594A. In experiments comparing various reference typing methods, the new primers provided 100% sensitivity and specificity for the O15:K52:H1 clonal group, including 162 diverse clinical and reference E. coli isolates.

Original languageEnglish (US)
Pages (from-to)3841-3843
Number of pages3
JournalJournal of clinical microbiology
Volume42
Issue number8
DOIs
StatePublished - Aug 2004

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