RAN translation at C9orf72-associated repeat expansions is selectively enhanced by the integrated stress response

Katelyn M. Green, M. Rebecca Glineburg, Michael G. Kearse, Brittany N. Flores, Alexander E. Linsalata, Stephen J. Fedak, Aaron C. Goldstrohm, Sami J. Barmada, Peter K. Todd

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Repeat-associated non-AUG (RAN) translation allows for unconventional initiation at disease-causing repeat expansions. As RAN translation contributes to pathogenesis in multiple neurodegenerative disorders, determining its mechanistic underpinnings may inform therapeutic development. Here we analyze RAN translation at G4C2 repeat expansions that cause C9orf72-associated amyotrophic lateral sclerosis and frontotemporal dementia (C9RAN) and at CGG repeats that cause fragile X-associated tremor/ataxia syndrome. We find that C9RAN translation initiates through a cap- and eIF4A-dependent mechanism that utilizes a CUG start codon. C9RAN and CGG RAN are both selectively enhanced by integrated stress response (ISR) activation. ISR-enhanced RAN translation requires an eIF2α phosphorylation-dependent alteration in start codon fidelity. In parallel, both CGG and G4C2 repeats trigger phosphorylated-eIF2α-dependent stress granule formation and global translational suppression. These findings support a model whereby repeat expansions elicit cellular stress conditions that favor RAN translation of toxic proteins, creating a potential feed-forward loop that contributes to neurodegeneration.

Original languageEnglish (US)
Article number2005
JournalNature Communications
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2017

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Initiator Codon
expansion
Poisons
Protein Biosynthesis
Neurodegenerative Diseases
Phosphorylation
ataxia
tremors
pathogenesis
phosphorylation
causes
Chemical activation
caps
actuators
retarding
activation
disorders
proteins
Proteins
Therapeutics

Cite this

Green, K. M., Glineburg, M. R., Kearse, M. G., Flores, B. N., Linsalata, A. E., Fedak, S. J., ... Todd, P. K. (2017). RAN translation at C9orf72-associated repeat expansions is selectively enhanced by the integrated stress response. Nature Communications, 8(1), [2005]. https://doi.org/10.1038/s41467-017-02200-0

RAN translation at C9orf72-associated repeat expansions is selectively enhanced by the integrated stress response. / Green, Katelyn M.; Glineburg, M. Rebecca; Kearse, Michael G.; Flores, Brittany N.; Linsalata, Alexander E.; Fedak, Stephen J.; Goldstrohm, Aaron C.; Barmada, Sami J.; Todd, Peter K.

In: Nature Communications, Vol. 8, No. 1, 2005, 01.12.2017.

Research output: Contribution to journalArticle

Green, KM, Glineburg, MR, Kearse, MG, Flores, BN, Linsalata, AE, Fedak, SJ, Goldstrohm, AC, Barmada, SJ & Todd, PK 2017, 'RAN translation at C9orf72-associated repeat expansions is selectively enhanced by the integrated stress response', Nature Communications, vol. 8, no. 1, 2005. https://doi.org/10.1038/s41467-017-02200-0
Green, Katelyn M. ; Glineburg, M. Rebecca ; Kearse, Michael G. ; Flores, Brittany N. ; Linsalata, Alexander E. ; Fedak, Stephen J. ; Goldstrohm, Aaron C. ; Barmada, Sami J. ; Todd, Peter K. / RAN translation at C9orf72-associated repeat expansions is selectively enhanced by the integrated stress response. In: Nature Communications. 2017 ; Vol. 8, No. 1.
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