Radionuclide assessment of myocardial injury in a heterotopic rat heart transplant model

C. G.A. McGregor, W. J. Hannan, D. J. Wheatley, A. F. Smith, A. L. Muir

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The use of heterotopic cardiac transplantation in rats of isogeneic strain has been investigated as a model of diffuse myocardial ischaemic injury. This model allowed the production of controlled graded injury prior to implantation of the donor heart in a recipient animal. In 21 animals the uptake of technetium-99m labelled pyrophosphate ([99mTc]PYP) in the transplanted heart, as measured by a γ-camera/computer system, agreed well with that determined by direct organ counting in a sodium iodide well-type detector (P < 0.001). The maximum uptake of [99mTc]PYP in the transplanted heart, as determined using the γ-camera, was found to be at 2 days after transplantation and 1 to 2 h after injection. For three groups each consisting of 25 rats the donor hearts were stored for 0, 30 or 90 min to produce a range of myocardial damage. For these groups there was no significant increase in creatine kinase with increasing storage time. For each group the uptake of [99mTc]PYP was significantly higher (P < 0.001) than for the previous storage time. The use of this model to investigate the long-term effects of myocardial injury is described.

Original languageEnglish (US)
Pages (from-to)189-194
Number of pages6
JournalInternational Journal of Nuclear Medicine and Biology
Issue number2
StatePublished - 1984
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgemenfs-The authors wish to thank Mrs D. Gray of the Department of Clinical Surgery, University of Edinburgh. Mr A. Millar of the Pharmacv Denartment. Royal &rmary, Edinburgh and Mrs M. Yeoman of the British Heart Foundation Research Unit, Papworth Hospital, Cambridge for their help in this study. This work was supported by a grant from the Scottish Hospitals Endowment Research Trust.


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