Radiometric prescreen antitumor activity with a Saccharomyces cerevisiae mutant strain

M. K. Speedie, D. V. Fique, R. N. Blomster

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


After modification, a technique for radiometrically measuring bacterial growth has been applied to a mutant strain of Saccharomyces cerevisiae. The assay is based on inhibition of 14CO2 release from [14C]glucose, which provides an extremely sensitive measure of cellular respiratory activity and growth. The criterion for antitumor activity is the differential inhibition of wild-type and mutant (distorted cell membrane) strains of the yeast. The system was optimized for medium, time of incubation, temperature, and size of inoculum. Known antitumor agents, including bleomycin, actinomycin D, adriamycin, and ellipticine were tested in the system, and differential inhibition was observed. Vincristine showed no inhibitory effects at the concentrations tried. The sensitivity for 20% inhibition ranged from 0.8 μg of adriamycin per ml to 0.14 mg of ellipticine per ml. Antifungal agents such as amphotericin B exhibited no differential inhibition. Antibacterial agents were inactive. This method may provide a rapid, sensitive, in vitro quantitative assay for antitumor agents which could be applied to a variety of assay needs and which can be run with facilities and equipment available in most laboratories.

Original languageEnglish (US)
Pages (from-to)171-175
Number of pages5
JournalAntimicrobial agents and chemotherapy
Issue number1
StatePublished - 1980


Dive into the research topics of 'Radiometric prescreen antitumor activity with a Saccharomyces cerevisiae mutant strain'. Together they form a unique fingerprint.

Cite this