Radioactive method for the assay of glycogen phosphorylases

D. P. Gilboe; K. L. Larson; F. Q. Nuttall

doi:10.1016/0003-2697(72)90274-6

Radioactive method for the assay of glycogen phosphorylases

D. P. Gilboe, K. L. Larson, F. Q. Nuttall

Medicine - Veteran's Administration Medical Center

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Abstract

A new assay for glycogen phosphorylase (EC 2.4.1.1) is presented. This assay employs a filter paper technique in which newly formed glycogen-¹⁴C is precipitated on paper squares using 66% ethanol while other radioactivity is removed by washing. It is rapid and reproducible and may be employed with crude as well as purified enzyme preparations. An additional advantage of the method is the potential for increased sensitivity. It should find utility for assay of extremely small tissue samples and should be useful in kinetic studies.

Original language	English (US)
Pages (from-to)	20-27
Number of pages	8
Journal	Analytical Biochemistry
Volume	47
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(72)90274-6
State	Published - May 1972

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abstract = "A new assay for glycogen phosphorylase (EC 2.4.1.1) is presented. This assay employs a filter paper technique in which newly formed glycogen-14C is precipitated on paper squares using 66% ethanol while other radioactivity is removed by washing. It is rapid and reproducible and may be employed with crude as well as purified enzyme preparations. An additional advantage of the method is the potential for increased sensitivity. It should find utility for assay of extremely small tissue samples and should be useful in kinetic studies.",

author = "Gilboe, {D. P.} and Larson, {K. L.} and Nuttall, {F. Q.}",

year = "1972",

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AU - Gilboe, D. P.

AU - Larson, K. L.

AU - Nuttall, F. Q.

PY - 1972/5

Y1 - 1972/5

N2 - A new assay for glycogen phosphorylase (EC 2.4.1.1) is presented. This assay employs a filter paper technique in which newly formed glycogen-14C is precipitated on paper squares using 66% ethanol while other radioactivity is removed by washing. It is rapid and reproducible and may be employed with crude as well as purified enzyme preparations. An additional advantage of the method is the potential for increased sensitivity. It should find utility for assay of extremely small tissue samples and should be useful in kinetic studies.

AB - A new assay for glycogen phosphorylase (EC 2.4.1.1) is presented. This assay employs a filter paper technique in which newly formed glycogen-14C is precipitated on paper squares using 66% ethanol while other radioactivity is removed by washing. It is rapid and reproducible and may be employed with crude as well as purified enzyme preparations. An additional advantage of the method is the potential for increased sensitivity. It should find utility for assay of extremely small tissue samples and should be useful in kinetic studies.

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

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Radioactive method for the assay of glycogen phosphorylases

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