Rack1 protects N-terminal phosphorylated c-Jun from Fbw7-mediated degradation

J. Zhang, F. Zhu, X. Li, Z. Dong, Y. Xu, C. Peng, S. Li, Y. Y. Cho, Ke Yao, Tatiana Zykova, A. M. Bode, Zigang Dong

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The c-Jun transcription factor is a highly unstable oncoprotein. Several ubiquitin ligases mediate c-Jun degradation. However, c-Jun can be stabilized once it is phosphorylated at the N-terminus by c-Jun N-terminal kinases (JNKs) or other protein kinases. This phosphorylation decreases c-Jun ubiquitination and degradation. The underlying mechanism for this phenomenon is still unknown. Here, we show that receptor for activated C-kinase 1 (Rack1) can bind with c-Jun and ubiquitin ligase Fbw7 to form a complex. When c-Jun is phosphorylated at the N-terminus, c-Jun is released from the complex and cannot be ubiquitinated by Fbw7, which leads to increased stabilization and accumulation of c-Jun. These results reveal that Rack1 has a very important role in tumorigenesis by maintaining the stability of c-Jun that has been phosphorylated at its N-terminus by JNKs or other kinases.

Original languageEnglish (US)
Pages (from-to)1835-1844
Number of pages10
JournalOncogene
Volume31
Issue number14
DOIs
StatePublished - Apr 5 2012

Bibliographical note

Funding Information:
We thank Dr Wang Luhai and Dr Ronai Ze’ev for providing the HA-FL-Rack1, HA-WD1-4-Rack1 and HA-WD5-7-Rack1 vectors and Dr Wei Wenyi for providing the Flag-wtc-Jun, Flag-DJNK-c-Jun, Flag-AF-c-Jun and Flag-AF-DJNK-c-Jun plasmids. We also thank Dr Bohmann Dirk for providing the c-JunAlaand c-JunAspmutant c-Jun plasmids. This study was supported by The Hormel Foundation and National Institutes of Health grants CA077646, CA111536, CA120388, ES016548 and R37 CA081064.

Keywords

  • Rack1
  • c-Jun
  • cell transformation
  • degradation
  • phosphorylation

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