Rac1 prevents cisplatin-induced apoptosis through down-regulation of p38 activation in NIH3T3 cells

Hye Gwang Jeong; Hyun Ju Cho; In Youb Chang; Sang Pil Yoon; Young Jin Jeon; Myung Hee Chung; Ho Jin You

doi:10.1016/S0014-5793(02)02674-1

Rac1 prevents cisplatin-induced apoptosis through down-regulation of p38 activation in NIH3T3 cells

Hye Gwang Jeong, Hyun Ju Cho, In Youb Chang, Sang Pil Yoon, Young Jin Jeon, Myung Hee Chung, Ho Jin You

Research output: Contribution to journal › Article › peer-review

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Abstract

In this study, the role of V12-Rac1 in the cisplatin-induced apoptosis was investigated. Cisplatin-induced apoptosis is associated with cytochrome c release, which can be inhibited by V12-Rac1 expression. The analysis of mitogen-activated protein kinase activity indicated that V12-Rac1 expression led to a decrease in p38 activity after exposure to cisplatin but not c-jun N-terminal kinase and extracellular signal-regulated kinase. Using pharmacological inhibitors, it was found that only p38 is a critical mediator in the cisplatin-induced apoptosis of NIH3T3 cells. This suggests that V12-Rac1 can stimulate the anti-apoptotic signaling pathway in response to cisplatin, and that decreased p38 activity caused by V12-Rac1 expression in cisplatin-treated NIH3T3 cells is crucial for V12-Rac1-dependent cell survival.

Original language	English (US)
Pages (from-to)	129-134
Number of pages	6
Journal	FEBS Letters
Volume	518
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(02)02674-1
State	Published - May 8 2002
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Ministry of Science and Technology, South Korea, and the KOSEF through the Research Center for Proteineous Materials. We thank Dr. D. Mercola (ISIS Pharmaceuticals, Carlsbad, CA, USA) for kindly providing us antisense oligonucleotides to JNK1 and JNK2 and scrambled oligonucleotide.

Keywords

Apoptosis
Cisplatin
Cytochrome c
Rac1
Rho family
p38

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10.1016/S0014-5793(02)02674-1

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title = "Rac1 prevents cisplatin-induced apoptosis through down-regulation of p38 activation in NIH3T3 cells",

abstract = "In this study, the role of V12-Rac1 in the cisplatin-induced apoptosis was investigated. Cisplatin-induced apoptosis is associated with cytochrome c release, which can be inhibited by V12-Rac1 expression. The analysis of mitogen-activated protein kinase activity indicated that V12-Rac1 expression led to a decrease in p38 activity after exposure to cisplatin but not c-jun N-terminal kinase and extracellular signal-regulated kinase. Using pharmacological inhibitors, it was found that only p38 is a critical mediator in the cisplatin-induced apoptosis of NIH3T3 cells. This suggests that V12-Rac1 can stimulate the anti-apoptotic signaling pathway in response to cisplatin, and that decreased p38 activity caused by V12-Rac1 expression in cisplatin-treated NIH3T3 cells is crucial for V12-Rac1-dependent cell survival.",

keywords = "Apoptosis, Cisplatin, Cytochrome c, Rac1, Rho family, p38",

author = "Jeong, {Hye Gwang} and Cho, {Hyun Ju} and Chang, {In Youb} and Yoon, {Sang Pil} and Jeon, {Young Jin} and Chung, {Myung Hee} and You, {Ho Jin}",

note = "Funding Information: This work was supported by the Ministry of Science and Technology, South Korea, and the KOSEF through the Research Center for Proteineous Materials. We thank Dr. D. Mercola (ISIS Pharmaceuticals, Carlsbad, CA, USA) for kindly providing us antisense oligonucleotides to JNK1 and JNK2 and scrambled oligonucleotide.",

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doi = "10.1016/S0014-5793(02)02674-1",

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T1 - Rac1 prevents cisplatin-induced apoptosis through down-regulation of p38 activation in NIH3T3 cells

AU - Jeong, Hye Gwang

AU - Cho, Hyun Ju

AU - Chang, In Youb

AU - Yoon, Sang Pil

AU - Jeon, Young Jin

AU - Chung, Myung Hee

AU - You, Ho Jin

N1 - Funding Information: This work was supported by the Ministry of Science and Technology, South Korea, and the KOSEF through the Research Center for Proteineous Materials. We thank Dr. D. Mercola (ISIS Pharmaceuticals, Carlsbad, CA, USA) for kindly providing us antisense oligonucleotides to JNK1 and JNK2 and scrambled oligonucleotide.

PY - 2002/5/8

Y1 - 2002/5/8

N2 - In this study, the role of V12-Rac1 in the cisplatin-induced apoptosis was investigated. Cisplatin-induced apoptosis is associated with cytochrome c release, which can be inhibited by V12-Rac1 expression. The analysis of mitogen-activated protein kinase activity indicated that V12-Rac1 expression led to a decrease in p38 activity after exposure to cisplatin but not c-jun N-terminal kinase and extracellular signal-regulated kinase. Using pharmacological inhibitors, it was found that only p38 is a critical mediator in the cisplatin-induced apoptosis of NIH3T3 cells. This suggests that V12-Rac1 can stimulate the anti-apoptotic signaling pathway in response to cisplatin, and that decreased p38 activity caused by V12-Rac1 expression in cisplatin-treated NIH3T3 cells is crucial for V12-Rac1-dependent cell survival.

AB - In this study, the role of V12-Rac1 in the cisplatin-induced apoptosis was investigated. Cisplatin-induced apoptosis is associated with cytochrome c release, which can be inhibited by V12-Rac1 expression. The analysis of mitogen-activated protein kinase activity indicated that V12-Rac1 expression led to a decrease in p38 activity after exposure to cisplatin but not c-jun N-terminal kinase and extracellular signal-regulated kinase. Using pharmacological inhibitors, it was found that only p38 is a critical mediator in the cisplatin-induced apoptosis of NIH3T3 cells. This suggests that V12-Rac1 can stimulate the anti-apoptotic signaling pathway in response to cisplatin, and that decreased p38 activity caused by V12-Rac1 expression in cisplatin-treated NIH3T3 cells is crucial for V12-Rac1-dependent cell survival.

KW - Apoptosis

KW - Cisplatin

KW - Cytochrome c

KW - Rac1

KW - Rho family

KW - p38

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ER -

Rac1 prevents cisplatin-induced apoptosis through down-regulation of p38 activation in NIH3T3 cells

Abstract

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