Quaternary structure of the liver microsomal cytochrome P-450

V. L. Tsuprun; K. N. Myasoedova; P. Berndt; O. N. Sograf; E. V. Orlova; V. Ya Chernyak; A. I. Archakov; V. P. Skulachev

doi:10.1016/0014-5793(86)80861-4

Quaternary structure of the liver microsomal cytochrome P-450

V. L. Tsuprun, K. N. Myasoedova, P. Berndt, O. N. Sograf, E. V. Orlova, V. Ya Chernyak, A. I. Archakov, V. P. Skulachev

Otolaryngology

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Abstract

Cytochrome P-450_LM2 was isolated from rabbit liver microsomes in a form which was shown to be homogeneous in AcA-22 Ultrogel and ultracentrifugation studies. The molecular mass determined by sedimentation equilibrium roughly corresponded to hexamer composed of 56 kDa monomers. Hexamer structure of the cytochrome was directly demonstrated by electron microscopic study. In the cytochrome P-450_LM2 hexamer, monomers seem to be arranged in two layers (three monomers in the layer) in such a way that each monomer occupies aposition at the vertices of a triangular antiprism with a 32 point group symmetry.

Original language	English (US)
Pages (from-to)	35-40
Number of pages	6
Journal	FEBS Letters
Volume	205
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(86)80861-4
State	Published - Sep 1 1986

Keywords

Cytochrome P-450
Electron microscopy
Quaternary structure

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10.1016/0014-5793(86)80861-4

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title = "Quaternary structure of the liver microsomal cytochrome P-450",

abstract = "Cytochrome P-450LM2 was isolated from rabbit liver microsomes in a form which was shown to be homogeneous in AcA-22 Ultrogel and ultracentrifugation studies. The molecular mass determined by sedimentation equilibrium roughly corresponded to hexamer composed of 56 kDa monomers. Hexamer structure of the cytochrome was directly demonstrated by electron microscopic study. In the cytochrome P-450LM2 hexamer, monomers seem to be arranged in two layers (three monomers in the layer) in such a way that each monomer occupies aposition at the vertices of a triangular antiprism with a 32 point group symmetry.",

keywords = "Cytochrome P-450, Electron microscopy, Quaternary structure",

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T1 - Quaternary structure of the liver microsomal cytochrome P-450

AU - Tsuprun, V. L.

AU - Myasoedova, K. N.

AU - Berndt, P.

AU - Sograf, O. N.

AU - Orlova, E. V.

AU - Chernyak, V. Ya

AU - Archakov, A. I.

AU - Skulachev, V. P.

PY - 1986/9/1

Y1 - 1986/9/1

N2 - Cytochrome P-450LM2 was isolated from rabbit liver microsomes in a form which was shown to be homogeneous in AcA-22 Ultrogel and ultracentrifugation studies. The molecular mass determined by sedimentation equilibrium roughly corresponded to hexamer composed of 56 kDa monomers. Hexamer structure of the cytochrome was directly demonstrated by electron microscopic study. In the cytochrome P-450LM2 hexamer, monomers seem to be arranged in two layers (three monomers in the layer) in such a way that each monomer occupies aposition at the vertices of a triangular antiprism with a 32 point group symmetry.

AB - Cytochrome P-450LM2 was isolated from rabbit liver microsomes in a form which was shown to be homogeneous in AcA-22 Ultrogel and ultracentrifugation studies. The molecular mass determined by sedimentation equilibrium roughly corresponded to hexamer composed of 56 kDa monomers. Hexamer structure of the cytochrome was directly demonstrated by electron microscopic study. In the cytochrome P-450LM2 hexamer, monomers seem to be arranged in two layers (three monomers in the layer) in such a way that each monomer occupies aposition at the vertices of a triangular antiprism with a 32 point group symmetry.

KW - Cytochrome P-450

KW - Electron microscopy

KW - Quaternary structure

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Quaternary structure of the liver microsomal cytochrome P-450

Abstract

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