Quantitative trait loci, epistasis and other interactions associated with dormancy in weedy rice (Oryza sativa l.)

M. E. Foley, X. Y. Gu, S. F. Kianian

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A wild weedy strain (i.e. SS18-2) of Oryza sativa L. was selected to investigate the geneticarchitecture underlying seed dormancy. A framework genetic map covering the whole ricegenome was constructed based on a backcross population (i.e. BC1 [EM93-1 (non-dormantbreeding line)//EM93-1/SS18-2]). Dormancy was determined by germination of seeds after1, 11 and 21 days after ripening (DAR). Six dormancy quantitative trait loci (QTLs), designatedas qSD4, 6, 7-1, 7-2, 8 and 12, were identified. The locus qSD7-1 was tightly linked tothe red pericarp colour gene Rc. In addition, the locus qSD4 was associated with an intervalimparting black hull colour. A QTL × DAR interaction was detected for qSD12, the locuswith the highest effect at 1, 11 and 21 DAR (R2 = 14%, 24% and 20%, respectively). Twoand more (i.e. three and four) orders of epistases were detected with four and all the sixQTLs, respectively. The higher-order epistases strongly suggest the presence of geneticallycomplex networks in the regulation of variation for seed dormancy in natural populations, and make it critical to select a favourable combination of alleles at multiple loci in positionalcloning of a target dormancy gene. Backcrossing techniques in combination with phenotypicand marker-assisted selection are being used to introduce the weedy rice-derived dormancylocus qSD7-1 into the EM93-1 genetic background to develop near isogenic lines for finemappingand positional cloning.

Original languageEnglish (US)
Title of host publicationSeeds
Subtitle of host publicationBiology, Development and Ecology
PublisherCABI Publishing
Pages217-224
Number of pages8
ISBN (Print)9781845931971
StatePublished - Feb 27 2007

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    Foley, M. E., Gu, X. Y., & Kianian, S. F. (2007). Quantitative trait loci, epistasis and other interactions associated with dormancy in weedy rice (Oryza sativa l.). In Seeds: Biology, Development and Ecology (pp. 217-224). CABI Publishing.