Quantitative T2 and T1ρ mapping are sensitive to ischemic injury to the epiphyseal cartilage in an in vivo piglet model of Legg-Calvé-Perthes disease

A. R. Armstrong; S. Bhave; Erick Buko; K. L. Chase; F. Tóth; C. S. Carlson; J. M. Ellermann; H. K.W. Kim; C. P. Johnson

doi:10.1016/j.joca.2022.05.009

Quantitative T2 and T1ρ mapping are sensitive to ischemic injury to the epiphyseal cartilage in an in vivo piglet model of Legg-Calvé-Perthes disease

A. R. Armstrong, S. Bhave, Erick Buko, K. L. Chase, F. Tóth, C. S. Carlson, J. M. Ellermann, H. K.W. Kim, C. P. Johnson

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Abstract

OBJECTIVE: To determine if the quantitative MRI techniques T2 and T1ρ mapping are sensitive to ischemic injury to epiphyseal cartilage in vivo in a piglet model of Legg-Calvé-Perthes disease using a clinical 3T MRI scanner. We hypothesized that T2 and T1ρ relaxation times would be increased in the epiphyseal cartilage of operated vs contralateral-control femoral heads 1 week following onset of ischemia.

DESIGN: Unilateral femoral head ischemia was surgically induced in eight piglets. Piglets were imaged 1 week post-operatively in vivo at 3T MRI using a magnetization-prepared 3D fast spin echo sequence for T2 and T1ρ mapping and a 3D gradient echo sequence for cartilage segmentation. Ischemia was confirmed in all piglets using gadolinium contrast-enhanced MRI. Median T2 and T1ρ relaxation times were measured in the epiphyseal cartilage of the ischemic and control femoral heads and compared using paired t-tests. Histological assessment was performed on a subset of five piglets.

RESULTS: T2 and T1ρ relaxation times were significantly increased in the epiphyseal cartilage of the operated vs control femoral heads (ΔT2 = 11.9 ± 3.7 ms, 95% CI = [8.8, 15.0] ms, P < 0.0001; ΔT1ρ = 12.8 ± 4.1 ms, 95% CI = [9.4, 16.2] ms, P < 0.0001). Histological assessment identified chondronecrosis in the hypertrophic and deep proliferative zones within ischemic epiphyseal cartilage.

CONCLUSIONS: T2 and T1ρ mapping are sensitive to ischemic injury to the epiphyseal cartilage in vivo at clinical 3T MRI. These techniques may be clinically useful to assess injury and repair to the epiphyseal cartilage to better stage the extent of ischemic damage in Legg-Calvé-Perthes disease.

Original language	English (US)
Pages (from-to)	1244-1253
Number of pages	10
Journal	Osteoarthritis and Cartilage
Volume	30
Issue number	9
DOIs	https://doi.org/10.1016/j.joca.2022.05.009
State	Published - Sep 2022

Bibliographical note

Funding Information:
This project was supported by grants from several institutes at the National Institutes of Health , including the National Institute of Arthritis and Musculoskeletal and Skin Diseases ( R56AR078315 , K01AR070894 , and T32AR050938 ), National Center for Advancing Translational Sciences ( UL1TR002494 ), Office of the Director ( K01OD021293 and T32OD010993 ), and National Institute of Biomedical Imaging and Bioengineering ( P41EB027061 ). The study sponsors had no role in the study design, collection, analysis and interpretation of data, the writing of the manuscript, or the decision to submit the manuscript for publication. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Publisher Copyright:
© 2022 Osteoarthritis Research Society International

Keywords

Epiphyseal cartilage
Ischemia
Legg-Calvé-Perthes disease
Magnetic resonance imaging
Necrosis
Relaxation time mapping

Center for Magnetic Resonance Research (CMRR) tags

NMC
MR
BI

PubMed: MeSH publication types

Journal Article
Research Support, N.I.H., Extramural

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Armstrong, A. R., Bhave, S., Buko, E., Chase, K. L., Tóth, F., Carlson, C. S., Ellermann, J. M., Kim, H. K. W., & Johnson, C. P. (2022). Quantitative T2 and T1ρ mapping are sensitive to ischemic injury to the epiphyseal cartilage in an in vivo piglet model of Legg-Calvé-Perthes disease. Osteoarthritis and Cartilage, 30(9), 1244-1253. https://doi.org/10.1016/j.joca.2022.05.009

Armstrong, AR, Bhave, S, Buko, E, Chase, KL, Tóth, F , Carlson, CS , Ellermann, JM, Kim, HKW & Johnson, CP 2022, 'Quantitative T2 and T1ρ mapping are sensitive to ischemic injury to the epiphyseal cartilage in an in vivo piglet model of Legg-Calvé-Perthes disease', Osteoarthritis and Cartilage, vol. 30, no. 9, pp. 1244-1253. https://doi.org/10.1016/j.joca.2022.05.009

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title = "Quantitative T2 and T1ρ mapping are sensitive to ischemic injury to the epiphyseal cartilage in an in vivo piglet model of Legg-Calv{\'e}-Perthes disease",

abstract = "OBJECTIVE: To determine if the quantitative MRI techniques T2 and T1ρ mapping are sensitive to ischemic injury to epiphyseal cartilage in vivo in a piglet model of Legg-Calv{\'e}-Perthes disease using a clinical 3T MRI scanner. We hypothesized that T2 and T1ρ relaxation times would be increased in the epiphyseal cartilage of operated vs contralateral-control femoral heads 1 week following onset of ischemia.DESIGN: Unilateral femoral head ischemia was surgically induced in eight piglets. Piglets were imaged 1 week post-operatively in vivo at 3T MRI using a magnetization-prepared 3D fast spin echo sequence for T2 and T1ρ mapping and a 3D gradient echo sequence for cartilage segmentation. Ischemia was confirmed in all piglets using gadolinium contrast-enhanced MRI. Median T2 and T1ρ relaxation times were measured in the epiphyseal cartilage of the ischemic and control femoral heads and compared using paired t-tests. Histological assessment was performed on a subset of five piglets.RESULTS: T2 and T1ρ relaxation times were significantly increased in the epiphyseal cartilage of the operated vs control femoral heads (ΔT2 = 11.9 ± 3.7 ms, 95% CI = [8.8, 15.0] ms, P < 0.0001; ΔT1ρ = 12.8 ± 4.1 ms, 95% CI = [9.4, 16.2] ms, P < 0.0001). Histological assessment identified chondronecrosis in the hypertrophic and deep proliferative zones within ischemic epiphyseal cartilage.CONCLUSIONS: T2 and T1ρ mapping are sensitive to ischemic injury to the epiphyseal cartilage in vivo at clinical 3T MRI. These techniques may be clinically useful to assess injury and repair to the epiphyseal cartilage to better stage the extent of ischemic damage in Legg-Calv{\'e}-Perthes disease.",

keywords = "Epiphyseal cartilage, Ischemia, Legg-Calv{\'e}-Perthes disease, Magnetic resonance imaging, Necrosis, Relaxation time mapping",

author = "Armstrong, {A. R.} and S. Bhave and Erick Buko and Chase, {K. L.} and F. T{\'o}th and Carlson, {C. S.} and Ellermann, {J. M.} and Kim, {H. K.W.} and Johnson, {C. P.}",

note = "Funding Information: This project was supported by grants from several institutes at the National Institutes of Health , including the National Institute of Arthritis and Musculoskeletal and Skin Diseases ( R56AR078315 , K01AR070894 , and T32AR050938 ), National Center for Advancing Translational Sciences ( UL1TR002494 ), Office of the Director ( K01OD021293 and T32OD010993 ), and National Institute of Biomedical Imaging and Bioengineering ( P41EB027061 ). The study sponsors had no role in the study design, collection, analysis and interpretation of data, the writing of the manuscript, or the decision to submit the manuscript for publication. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Publisher Copyright: {\textcopyright} 2022 Osteoarthritis Research Society International",

year = "2022",

month = sep,

doi = "10.1016/j.joca.2022.05.009",

language = "English (US)",

volume = "30",

pages = "1244--1253",

journal = "Osteoarthritis and Cartilage",

issn = "1063-4584",

publisher = "W.B. Saunders Ltd",

number = "9",

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TY - JOUR

T1 - Quantitative T2 and T1ρ mapping are sensitive to ischemic injury to the epiphyseal cartilage in an in vivo piglet model of Legg-Calvé-Perthes disease

AU - Armstrong, A. R.

AU - Bhave, S.

AU - Buko, Erick

AU - Chase, K. L.

AU - Tóth, F.

AU - Carlson, C. S.

AU - Ellermann, J. M.

AU - Kim, H. K.W.

AU - Johnson, C. P.

N1 - Funding Information: This project was supported by grants from several institutes at the National Institutes of Health , including the National Institute of Arthritis and Musculoskeletal and Skin Diseases ( R56AR078315 , K01AR070894 , and T32AR050938 ), National Center for Advancing Translational Sciences ( UL1TR002494 ), Office of the Director ( K01OD021293 and T32OD010993 ), and National Institute of Biomedical Imaging and Bioengineering ( P41EB027061 ). The study sponsors had no role in the study design, collection, analysis and interpretation of data, the writing of the manuscript, or the decision to submit the manuscript for publication. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Publisher Copyright: © 2022 Osteoarthritis Research Society International

PY - 2022/9

Y1 - 2022/9

N2 - OBJECTIVE: To determine if the quantitative MRI techniques T2 and T1ρ mapping are sensitive to ischemic injury to epiphyseal cartilage in vivo in a piglet model of Legg-Calvé-Perthes disease using a clinical 3T MRI scanner. We hypothesized that T2 and T1ρ relaxation times would be increased in the epiphyseal cartilage of operated vs contralateral-control femoral heads 1 week following onset of ischemia.DESIGN: Unilateral femoral head ischemia was surgically induced in eight piglets. Piglets were imaged 1 week post-operatively in vivo at 3T MRI using a magnetization-prepared 3D fast spin echo sequence for T2 and T1ρ mapping and a 3D gradient echo sequence for cartilage segmentation. Ischemia was confirmed in all piglets using gadolinium contrast-enhanced MRI. Median T2 and T1ρ relaxation times were measured in the epiphyseal cartilage of the ischemic and control femoral heads and compared using paired t-tests. Histological assessment was performed on a subset of five piglets.RESULTS: T2 and T1ρ relaxation times were significantly increased in the epiphyseal cartilage of the operated vs control femoral heads (ΔT2 = 11.9 ± 3.7 ms, 95% CI = [8.8, 15.0] ms, P < 0.0001; ΔT1ρ = 12.8 ± 4.1 ms, 95% CI = [9.4, 16.2] ms, P < 0.0001). Histological assessment identified chondronecrosis in the hypertrophic and deep proliferative zones within ischemic epiphyseal cartilage.CONCLUSIONS: T2 and T1ρ mapping are sensitive to ischemic injury to the epiphyseal cartilage in vivo at clinical 3T MRI. These techniques may be clinically useful to assess injury and repair to the epiphyseal cartilage to better stage the extent of ischemic damage in Legg-Calvé-Perthes disease.

AB - OBJECTIVE: To determine if the quantitative MRI techniques T2 and T1ρ mapping are sensitive to ischemic injury to epiphyseal cartilage in vivo in a piglet model of Legg-Calvé-Perthes disease using a clinical 3T MRI scanner. We hypothesized that T2 and T1ρ relaxation times would be increased in the epiphyseal cartilage of operated vs contralateral-control femoral heads 1 week following onset of ischemia.DESIGN: Unilateral femoral head ischemia was surgically induced in eight piglets. Piglets were imaged 1 week post-operatively in vivo at 3T MRI using a magnetization-prepared 3D fast spin echo sequence for T2 and T1ρ mapping and a 3D gradient echo sequence for cartilage segmentation. Ischemia was confirmed in all piglets using gadolinium contrast-enhanced MRI. Median T2 and T1ρ relaxation times were measured in the epiphyseal cartilage of the ischemic and control femoral heads and compared using paired t-tests. Histological assessment was performed on a subset of five piglets.RESULTS: T2 and T1ρ relaxation times were significantly increased in the epiphyseal cartilage of the operated vs control femoral heads (ΔT2 = 11.9 ± 3.7 ms, 95% CI = [8.8, 15.0] ms, P < 0.0001; ΔT1ρ = 12.8 ± 4.1 ms, 95% CI = [9.4, 16.2] ms, P < 0.0001). Histological assessment identified chondronecrosis in the hypertrophic and deep proliferative zones within ischemic epiphyseal cartilage.CONCLUSIONS: T2 and T1ρ mapping are sensitive to ischemic injury to the epiphyseal cartilage in vivo at clinical 3T MRI. These techniques may be clinically useful to assess injury and repair to the epiphyseal cartilage to better stage the extent of ischemic damage in Legg-Calvé-Perthes disease.

KW - Epiphyseal cartilage

KW - Ischemia

KW - Legg-Calvé-Perthes disease

KW - Magnetic resonance imaging

KW - Necrosis

KW - Relaxation time mapping

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DO - 10.1016/j.joca.2022.05.009

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C2 - 35644462

AN - SCOPUS:85131821427

SN - 1063-4584

VL - 30

SP - 1244

EP - 1253

JO - Osteoarthritis and Cartilage

JF - Osteoarthritis and Cartilage

IS - 9

ER -

Quantitative T2 and T1ρ mapping are sensitive to ischemic injury to the epiphyseal cartilage in an in vivo piglet model of Legg-Calvé-Perthes disease

Abstract

Bibliographical note

Keywords

Center for Magnetic Resonance Research (CMRR) tags

PubMed: MeSH publication types

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