Quantitative profiling of feruloylated arabinoxylan side-chains from graminaceous cell walls.

R. R. Schendel, M. R. Meyer, M. Bunzel

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Graminaceous arabinoxylans are distinguished by decoration with feruloylated monosaccharidic and oligosaccharidic side-chains. Although it is hypothesized that structural complexity and abundance of these feruloylated arabinoxylan side-chains may contribute, among other factors, to resistance of plant cell walls to enzymatic degradation, quantitative profiling approaches for these structural units in plant cell wall materials have not been described yet. Here we report the development and application of a rapid and robust method enabling the quantitative comparison of feruloylated side-chain profiles in cell wall materials following mildly acidic hydrolysis, C18-solid phase extraction (SPE), reduction under aprotic conditions, and liquid chromatography with diode-array detection/mass spectrometry (LC-DAD/MS) separation and detection. The method was applied to the insoluble fiber/cell wall materials isolated from 12 whole grains: wild rice (Zizania aquatica L.), long-grain brown rice (Oryza sativa L.), rye (Secale cereale L.), kamut (Triticum turanicum Jakubz.), wheat (Triticum aestivum L.), spelt (Triticum spelta L.), intermediate wheatgrass (Thinopyrum intermedium), maize (Zea mays L.), popcorn (Zea mays L. var. everta), oat (Avena sativa L.) (dehulled), barley (Hordeum vulgare L.) (dehulled), and proso millet (Panicum miliaceum L.). Between 51 and 96% of the total esterified monomeric ferulates were represented in the quantified compounds captured in the feruloylated side-chain profiles, which confirms the significance of these structures to the global arabinoxylan structure in terms of quantity. The method provided new structural insights into cereal grain arabinoxylans, in particular, that the structural moiety alpha -L-galactopyranosyl-(1->2)- beta -D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose (FAXG), which had previously only been described in maize, is ubiquitous to cereal grains.
Original languageEnglish (US)
JournalFrontiers in Plant Science
Volume7
Issue numberJanuary
StatePublished - 2016

Keywords

  • methodology
  • chemical composition
  • rice
  • wheat
  • barley
  • maize
  • wild relatives
  • cell wall components
  • cell walls
  • oats
  • plant composition
  • rye
  • Biochemistry and Molecular Biophysics
  • feruloylated arabinoxylan side-chain profiling
  • laboratory techniques, spectrum analysis techniques, chromatographic techniques
  • laboratory techniques/C18-solid phase extraction
  • laboratory techniques/liquid chromatography-diode array detection/mass spectrometry
  • Membranes (Cell Biology)
  • Methods and Techniques

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