Quantitative PCR for tracking the megaplasmid-borne biodegradation potential of a model sphingomonad

Erica M. Hartmann; Jonathan P. Badalamenti; Rosa Krajmalnik-Brown; Rolf U. Halden

doi:10.1128/AEM.00715-12

Quantitative PCR for tracking the megaplasmid-borne biodegradation potential of a model sphingomonad

Erica M. Hartmann
, Jonathan P. Badalamenti
, Rosa Krajmalnik-Brown
, Rolf U. Halden

Genomics Center

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We developed a quantitative PCR method for tracking the dxnA1 gene, the initial, megaplasmid-borne gene in Sphingomonas wittichii RW1's dibenzo-p-dioxin degradation pathway. We used this method on complex environmental samples and report on growth of S. wittichii RW1 in landfill leachate, thus furnishing a novel tool for monitoring megaplasmid-borne, dioxygenaseencoding genes.

Original language	English (US)
Pages (from-to)	4493-4496
Number of pages	4
Journal	Applied and environmental microbiology
Volume	78
Issue number	12
DOIs	https://doi.org/10.1128/AEM.00715-12
State	Published - Jun 2012

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SDG 12 Responsible Consumption and Production

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abstract = "We developed a quantitative PCR method for tracking the dxnA1 gene, the initial, megaplasmid-borne gene in Sphingomonas wittichii RW1's dibenzo-p-dioxin degradation pathway. We used this method on complex environmental samples and report on growth of S. wittichii RW1 in landfill leachate, thus furnishing a novel tool for monitoring megaplasmid-borne, dioxygenaseencoding genes.",

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AU - Halden, Rolf U.

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AB - We developed a quantitative PCR method for tracking the dxnA1 gene, the initial, megaplasmid-borne gene in Sphingomonas wittichii RW1's dibenzo-p-dioxin degradation pathway. We used this method on complex environmental samples and report on growth of S. wittichii RW1 in landfill leachate, thus furnishing a novel tool for monitoring megaplasmid-borne, dioxygenaseencoding genes.

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ER -

Quantitative PCR for tracking the megaplasmid-borne biodegradation potential of a model sphingomonad

Abstract

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