Quantitative PCR for tracking the megaplasmid-borne biodegradation potential of a model sphingomonad

Erica M. Hartmann, Jonathan P. Badalamenti, Rosa Krajmalnik-Brown, Rolf U. Halden

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

We developed a quantitative PCR method for tracking the dxnA1 gene, the initial, megaplasmid-borne gene in Sphingomonas wittichii RW1's dibenzo-p-dioxin degradation pathway. We used this method on complex environmental samples and report on growth of S. wittichii RW1 in landfill leachate, thus furnishing a novel tool for monitoring megaplasmid-borne, dioxygenaseencoding genes.

Original languageEnglish (US)
Pages (from-to)4493-4496
Number of pages4
JournalApplied and environmental microbiology
Volume78
Issue number12
DOIs
StatePublished - Jun 2012

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