Quantitative PCR for measuring biomass of decomposer fungi in planta

Zewei Song, Andrew Vail, Michael J Sadowsky, Jonathan S Schilling

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The biomass of brown and white rot fungi were estimated using ergosterol and quantitative PCR (qPCR). Each biomass estimate was compared with biomass measured gravimetrically from liquid cultures, as well as from three wood substrata at two decay stages. Fungal morphological changes in two different substrata, agar and pine, were measured using a chitin-specific fluorophore and confocal microscopy. In liquid culture, the two fungal isolates had significantly different biomass conversion factors for both methods. In wood colonized for 3wks, qPCR yielded a lower estimate than did ergosterol, while at week 8 it yielded a higher estimate. Changes in average fungal cell dimensions partially explained differences between the two methods. Overall, our results suggest that a constant conversion factor cannot be assumed as default for either method. Instead, it demonstrates the importance of standardizing wood species, decay class, fungus-specific conversion factor, and DNA extraction protocol in order to properly estimate fungal biomass in woody substrata.

Original languageEnglish (US)
Pages (from-to)39-46
Number of pages8
JournalFungal Ecology
Volume7
Issue number1
DOIs
StatePublished - Feb 2014

Bibliographical note

Funding Information:
This research was made possible through the generous support of the Conservation and the Environment grants program of The Andrew W. Mellon Foundation (New York, NY) . We would like to thank Adam Vogel and Mark Sanders from University of Minnesota Imaging Center for help with confocal microscopy. We also want to thank Benjamin Held and Dr. Robert Blanchette for help with thin-sectioning.

Keywords

  • Biomass
  • Ergosterol
  • Fungi
  • Quantitative PCR
  • Wood decomposition

Fingerprint

Dive into the research topics of 'Quantitative PCR for measuring biomass of decomposer fungi in planta'. Together they form a unique fingerprint.

Cite this