Quantitative optical trapping on single organelles in cell extract

Pradeep Barak, Ashim Rai, Priyanka Rai, Roop Mallik

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

We have developed an optical trapping method to precisely measure the force generated by motor proteins on single organelles of unknown size in cell extract. This approach, termed VMatch, permits the functional interrogation of native motor complexes. We apply VMatch to measure the force, number and activity of kinesin-1 on motile lipid droplets isolated from the liver of normally fed and food-deprived rats.

Original languageEnglish (US)
Pages (from-to)68-70
Number of pages3
JournalNature Methods
Volume10
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint Dive into the research topics of 'Quantitative optical trapping on single organelles in cell extract'. Together they form a unique fingerprint.

Cite this