Quantitative optical trapping on single organelles in cell extract

Pradeep Barak; Ashim Rai; Priyanka Rai; Roop Mallik

doi:10.1038/nmeth.2287

Quantitative optical trapping on single organelles in cell extract

Pradeep Barak, Ashim Rai, Priyanka Rai, Roop Mallik

Genetics, Cell Biology and Development (CBS)

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

We have developed an optical trapping method to precisely measure the force generated by motor proteins on single organelles of unknown size in cell extract. This approach, termed VMatch, permits the functional interrogation of native motor complexes. We apply VMatch to measure the force, number and activity of kinesin-1 on motile lipid droplets isolated from the liver of normally fed and food-deprived rats.

Original language	English (US)
Pages (from-to)	68-70
Number of pages	3
Journal	Nature Methods
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/nmeth.2287
State	Published - Jan 2013

Bibliographical note

Funding Information:
We thank A. Rai and R. Jha for discussions and help and K. Verhey (University of Michigan) for kinesin-1 antibody. R.M. acknowledges funding through an International Senior Research Fellowship from the Wellcome Trust, UK (grant WT079214MA).

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author = "Pradeep Barak and Ashim Rai and Priyanka Rai and Roop Mallik",

note = "Funding Information: We thank A. Rai and R. Jha for discussions and help and K. Verhey (University of Michigan) for kinesin-1 antibody. R.M. acknowledges funding through an International Senior Research Fellowship from the Wellcome Trust, UK (grant WT079214MA).",

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AU - Rai, Priyanka

AU - Mallik, Roop

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N2 - We have developed an optical trapping method to precisely measure the force generated by motor proteins on single organelles of unknown size in cell extract. This approach, termed VMatch, permits the functional interrogation of native motor complexes. We apply VMatch to measure the force, number and activity of kinesin-1 on motile lipid droplets isolated from the liver of normally fed and food-deprived rats.

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Quantitative optical trapping on single organelles in cell extract

Abstract

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