Quantitative measurement of 4-hydroxyalkenals in oxidized low-density lipoprotein by gas chromatography-mass spectrometry

Frederik J.G.M. Vankuijk, Aristotle N. Siakotos, Loren G. Fong, Robert J. Stephens, David W. Thomas

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Previously, we reported on the determination of 4-hydroxyalkenals as pentafluorobenzyl oxime derivatives by gas chromatography-mass spectrometry. In this study, an improved method is presented which allows quantitative detection of 4-hydroxyalkenals in tissues by using two stable isotope-labeled internal standards, 9D3-4-hydroxynonenal and 9D3-4-hydroxyhexenal. This assay was used to quantitate 4-hydroxyalkenals in copper-oxidized human low-density lipoprotein, and we found more 4-hydroxynonenal than 4-hydroxyhexenal. This is consistent with the fact that there are more ω-6 than ω-3 fatty acids in human low-density lipoprotein samples, which are the sources of 4-hydroxynonenal and 4-hydroxyhexenal, respectively.

Original languageEnglish (US)
Pages (from-to)420-424
Number of pages5
JournalAnalytical Biochemistry
Volume224
Issue number1
DOIs
StatePublished - Jan 1995
Externally publishedYes

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