Quantitative imaging of protein-protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera

R. V. Krishnan, A. Masuda, V. E. Centonze, B. Herman

Research output: Contribution to journalReview articlepeer-review

69 Scopus citations

Abstract

Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM system using a streak camera is described. An example of a typical application of the system is provided in which the fluorescence resonance energy transfer between a donor-acceptor pair of fluorescent proteins within a cellular specimen is measured.

Original languageEnglish (US)
Pages (from-to)362-367
Number of pages6
JournalJournal of biomedical optics
Volume8
Issue number3
DOIs
StatePublished - Jul 2003

Keywords

  • Fluorescence lifetime imaging microscopy

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