Quantitative image analysis of HIV-1 infection in lymphoid tissue

Ashley T. Haase, Keith Henry, Mary Zupancic, Gerald Sedgewick, Russell A. Faust, Holly Melroe, Winston Cavert, Kristin Gebhard, Katherine Staskus, Zhi Qiang Zhang, Peter J. Dailey, Henry H. Balfour, Alejo Erice, Alan S. Perelson

Research output: Contribution to journalArticlepeer-review

489 Scopus citations

Abstract

Tracking human immunodeficiency virus-type 1 (HIV-1) infection at the cellular level in tissue reservoirs provides opportunities to better understand the pathogenesis of infection and to rationally design and monitor therapy. A quantitative technique was developed to determine viral burden in two important cellular compartments in lymphoid tissues. Image analysis and in situ hybridization were combined to show that in the presymptomatic stages of infection there is a large, relatively stable pool of virions on the surfaces of follicular dendritic cells and a smaller pool of productively infected cells. Despite evidence of constraints on HIV-1 replication in the infected cell population in lymphoid tissues, estimates of the numbers of these cells and the virus they could produce are consistent with the quantities of virus that have been detected in the bloodstream. The cellular sources of virus production and storage in lymphoid tissues can now be studied with this approach over the course of infection and treatment.

Original languageEnglish (US)
Pages (from-to)985-989
Number of pages5
JournalScience
Volume274
Issue number5289
DOIs
StatePublished - Nov 8 1996

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