Quantitative autoradiographic analysis of [125I]-human CGRP binding sites in the dorsal horn of rat following chronic constriction injury or dorsal rhizotomy

Mary G Garry, Keith C. Kajander, Gary J. Bennett, Virginia S. Seybold

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Quantitative receptor autoradiography was used to examine the binding of [125I]-human CGRP in the dorsal horn of the L4 spinal segment of rats with a chronic constriction injury (CCI) of the sciatic nerve or unilateral dorsal rhizotomies of spinal segments L1-L6. At the times selected for study, we found no change in the amount of CGRP binding in any areas examined following CCI. In contrast, our results showed a temporally related increase in the amount of CGRP binding in areas within laminae I-II and in lateral lamina V of the dorsal horn ipsilateral to the rhizotomies. These results indicate that CGRP binding sites are regulated, most likely, by changes in the release of CGRP. Further, our results suggest that the release of CGRP from primary afferent neurons is unchanged in animals with a CCI.

Original languageEnglish (US)
Pages (from-to)1365-1373
Number of pages9
JournalPeptides
Volume12
Issue number6
DOIs
StatePublished - Jan 1 1991

Keywords

  • Binding sites
  • CGRP
  • Chronic constriction injury
  • Dorsal horn
  • Dorsal rhizotomy

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