Quantitative assessment of cochlear and vestibular ganglion neurons in temporal bones with chronic otitis media

Rafael da Costa Monsanto, Norma de Oliveira Penido, Mio Uchiyama, Patricia Schachern, Michael M. Paparella, Sebahattin Cureoglu

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: In this study, we aimed to determine whether or not COM leads to loss of spiral and Scarpa ganglion neurons. Methods: From the human temporal bone (HTB) collection at the University of Minnesota we selected human temporal bones with COM, defined as the presence of clinically intractable tissue abnormalities in the middle ear (cholesteatoma, perforation of the eardrum, granulation tissue, fibrosis, tympanosclerosis, and cholesterol granuloma). We also selected HTBs from donors with no ear diseases as controls. We quantitatively analyzed the number of spiral and Scarpa ganglion cells and compared the results obtained in the control and study groups. Results: In both COM and control groups we observed a significant negative correlation between age and number of both spiral (R = -0.632; P < 0.001; 95% CI − 0.766 to − 0.434) and Scarpa ganglion (R = − 0.404; P = 0.008; 95% CI − 0.636 to − 0.051) cells. We did not find any significant differences in the number of spiral ganglion cells (in total or per segment) or in the density of Scarpa ganglion cells (in each vestibular nerve or both) in the COM group as compared with controls (P > 0.05). Conclusions and relevance: Our results did not demonstrate significant loss of cochlear or vestibular peripheral ganglion neuron loss in HTBs with COM as compared with controls.

Original languageEnglish (US)
JournalEuropean Archives of Oto-Rhino-Laryngology
DOIs
StateAccepted/In press - 2020

Bibliographical note

Funding Information:
RCM received a scholarship from the “Coordenação de aperfeiçoamento pessoal de nível superior” (CAPES) (Finance code: 001). The research reported in this manuscript was supported by the National Institute of Neurological Disorders and Stroke of the NIH (UG3NS107688), the International Hearing Foundation, the 5 M Lions International, and the Starkey Foundation. Acknowledgements

Funding Information:
We thank our supporters: the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES—Brazil) (Finance code: 001), the National Institute of Neurological Disorders and Stroke of the NIH (Grant No. UG3NS107688), the International Hearing Foundation, the 5 M Lions International, and the Starkey Foundation.

Keywords

  • Chronic otitis media
  • Hearing loss
  • Otitis media
  • Scarpa ganglion cells
  • Spiral ganglion cells
  • Tinnitus
  • Vestibular diseases

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