Quantitative assessment of brain glucose metabolic rates using in vivo deuterium magnetic resonance spectroscopy

Ming Lu, Xiao-Hong Zhu, Yi Zhang, Gheorghe Mateescu, Wei Chen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Quantitative assessment of cerebral glucose consumption rate (CMRglc) and tricarboxylic acid cycle flux (VTCA) is crucial for understanding neuroenergetics under physiopathological conditions. In this study, we report a novel in vivo Deuterium (2H) MRS (DMRS) approach for simultaneously measuring and quantifying CMRglc and VTCA in rat brains at 16.4 Tesla. Following a brief infusion of deuterated glucose, dynamic changes of isotope-labeled glucose, glutamate/glutamine (Glx) and water contents in the brain can be robustly monitored from their well-resolved 2H resonances. Dynamic DMRS glucose and Glx data were employed to determine CMRglc and VTCA concurrently. To test the sensitivity of this method in response to altered glucose metabolism, two brain conditions with different anesthetics were investigated. Increased CMRglc (0.46 vs. 0.28 µmol/g/min) and VTCA (0.96 vs. 0.6 µmol/g/min) were found in rats under morphine as compared to deeper anesthesia using 2% isoflurane. This study demonstrates the feasibility and new utility of the in vivo DMRS approach to assess cerebral glucose metabolic rates at high/ultrahigh field. It provides an alternative MRS tool for in vivo study of metabolic coupling relationship between aerobic and anaerobic glucose metabolisms in brain under physiopathological states.

Original languageEnglish (US)
Pages (from-to)3518-3530
Number of pages13
JournalJournal of Cerebral Blood Flow and Metabolism
Volume37
Issue number11
DOIs
StatePublished - Nov 1 2017

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Deuterium
Magnetic Resonance Spectroscopy
Glucose
Brain
Anaerobiosis
Citric Acid Cycle
Isoflurane
Feasibility Studies
Glutamine
Isotopes
Morphine
Anesthetics
Glutamic Acid
Anesthesia
Water

Keywords

  • Brain glucose metabolisms
  • TCA cycle
  • cerebral metabolic rate of glucose
  • deuterium magnetic resonance spectroscopy (H MRS)
  • glycolysis

PubMed: MeSH publication types

  • Journal Article

Cite this

Quantitative assessment of brain glucose metabolic rates using in vivo deuterium magnetic resonance spectroscopy. / Lu, Ming; Zhu, Xiao-Hong; Zhang, Yi; Mateescu, Gheorghe; Chen, Wei.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 37, No. 11, 01.11.2017, p. 3518-3530.

Research output: Contribution to journalArticle

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