Quantitative and real-time detection of secretion of chemical messengers from individual platelets

Shencheng Ge, Nathan J. Wittenberg, Christy L. Haynes

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Carbon-fiber microelectrochemical methods were utilized in this study to measure individual exocytotic events of secretion of serotonin and histamine from washed rabbit platelets. The quantal release of serotonin was quantitatively characterized with a δ-granule serotonin concentration of 0.6 M and secretion time course of 7 ms. Additionally, extracellular osmolarity influences quantal size, causing quantal size increases under hypotonic conditions, presumably due to the influx of cytosolic serotonin into the halo region of the δ-granules.

Original languageEnglish (US)
Pages (from-to)7020-7024
Number of pages5
JournalBiochemistry
Volume47
Issue number27
DOIs
StatePublished - Jul 8 2008

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