Quantitative analysis of mRNA for 10 different drug resistance factors in dogs with lymphoma

Hirotaka Tomiyasu, Yuko Goto-Koshino, Masashi Takahashi, Yasuhito Fujino, Koichi Ohno, Hajime Tsujimoto

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31 Scopus citations


Expression levels of ABCB1, ABCC1, Lung resistance-associated protein (LRP), ABCG2, p53, p21waf1, Bcl-2, CD40L, glutathione S-transferase alpha (GSTα), and O6-methylguanine-DNA-methyltransferase (MGMT) genes, and mutation of p53 gene were examined in 23 dogs with multicentric high-grade lymphoma to explore their association with drug resistance of the tumor cells. Dogs were divided into chemotherapy-sensitive (n=13) and -resistant (n=10) groups according to the response to a 6-month modified version of the University of Wisconsin (UW)-Madison chemotherapy protocol (UW-25), and expression levels of these factors and frequency of p53 gene mutation were compared between groups. No significant differences were observed in expression levels of each factor between groups. However, 4 dogs in the chemotherapy-resistant group showed high expression of ABCB1. No significant difference was observed in the frequency of p53 mutation between groups. A possible association of ABCB1 with resistance to UW-25 was shown, but no uniform mechanism associated with drug resistance could be identified in dogs with lymphoma.

Original languageEnglish (US)
Pages (from-to)1165-1172
Number of pages8
JournalJournal of Veterinary Medical Science
Issue number9
StatePublished - Sep 2010


  • Canine
  • Drug resistance
  • Lymphoma
  • Real-time RT-PCR
  • Sequence analysis


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