Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with a bovine rotavirus VP6 experimental vaccine

D. D. Gonzalez, A. Rimondi, M. S. Perez Aguirreburualde, M. Mozgovoj, D. Bellido, A. Wigdorovitz, M. J. Dus Santos

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing neutralizing antibodies (NAb), suggesting that another immunological effectors were involved in the protection observed. In this work, groups of cows (n= 4) were immunized in the last third of gestation with a bovine rotavirus (BRV) experimental vaccine and with a VP6 subunit vaccine. At birth, colostrums from vaccinated and non-vaccinated cows were processed and viable colostral mononuclear cells were obtained. With the purpose of determining the cytokine patterns generated by cells from immune secretions (colostrums and milk), a relative quantification by real time PCR was standardized. Quantitative real time PCR (qPCR) was used to determine transcript levels of IL-4, IL-6, IL-10, IL-12, IFN-γ and IFN-α from these cells. Colostral and milk mononuclear cells expressed a different cytokine transcript expression pattern regarding the vaccine used. These results demonstrated that the colostral cellular population was active and could exert its action influencing the final immune response.

Original languageEnglish (US)
Pages (from-to)703-708
Number of pages6
JournalResearch in veterinary science
Volume95
Issue number2
DOIs
StatePublished - Oct 1 2013

Fingerprint

cow colostrum
Colostrum
Rotavirus
Real-Time Polymerase Chain Reaction
Milk
quantitative polymerase chain reaction
cytokines
Vaccines
Cytokines
vaccines
Gene Expression
cows
milk
gene expression
cattle
calves
cells
Rotavirus Vaccines
Subunit Vaccines
subunit vaccines

Keywords

  • Colostrum cells
  • QPCR relative method
  • VP6 subunit vaccine

Cite this

Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with a bovine rotavirus VP6 experimental vaccine. / Gonzalez, D. D.; Rimondi, A.; Perez Aguirreburualde, M. S.; Mozgovoj, M.; Bellido, D.; Wigdorovitz, A.; Dus Santos, M. J.

In: Research in veterinary science, Vol. 95, No. 2, 01.10.2013, p. 703-708.

Research output: Contribution to journalArticle

@article{ee16b5785df24bb4ad711b626be02ec0,
title = "Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with a bovine rotavirus VP6 experimental vaccine",
abstract = "In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing neutralizing antibodies (NAb), suggesting that another immunological effectors were involved in the protection observed. In this work, groups of cows (n= 4) were immunized in the last third of gestation with a bovine rotavirus (BRV) experimental vaccine and with a VP6 subunit vaccine. At birth, colostrums from vaccinated and non-vaccinated cows were processed and viable colostral mononuclear cells were obtained. With the purpose of determining the cytokine patterns generated by cells from immune secretions (colostrums and milk), a relative quantification by real time PCR was standardized. Quantitative real time PCR (qPCR) was used to determine transcript levels of IL-4, IL-6, IL-10, IL-12, IFN-γ and IFN-α from these cells. Colostral and milk mononuclear cells expressed a different cytokine transcript expression pattern regarding the vaccine used. These results demonstrated that the colostral cellular population was active and could exert its action influencing the final immune response.",
keywords = "Colostrum cells, QPCR relative method, VP6 subunit vaccine",
author = "Gonzalez, {D. D.} and A. Rimondi and {Perez Aguirreburualde}, {M. S.} and M. Mozgovoj and D. Bellido and A. Wigdorovitz and {Dus Santos}, {M. J.}",
year = "2013",
month = "10",
day = "1",
doi = "10.1016/j.rvsc.2013.03.016",
language = "English (US)",
volume = "95",
pages = "703--708",
journal = "Research in Veterinary Science",
issn = "0034-5288",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with a bovine rotavirus VP6 experimental vaccine

AU - Gonzalez, D. D.

AU - Rimondi, A.

AU - Perez Aguirreburualde, M. S.

AU - Mozgovoj, M.

AU - Bellido, D.

AU - Wigdorovitz, A.

AU - Dus Santos, M. J.

PY - 2013/10/1

Y1 - 2013/10/1

N2 - In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing neutralizing antibodies (NAb), suggesting that another immunological effectors were involved in the protection observed. In this work, groups of cows (n= 4) were immunized in the last third of gestation with a bovine rotavirus (BRV) experimental vaccine and with a VP6 subunit vaccine. At birth, colostrums from vaccinated and non-vaccinated cows were processed and viable colostral mononuclear cells were obtained. With the purpose of determining the cytokine patterns generated by cells from immune secretions (colostrums and milk), a relative quantification by real time PCR was standardized. Quantitative real time PCR (qPCR) was used to determine transcript levels of IL-4, IL-6, IL-10, IL-12, IFN-γ and IFN-α from these cells. Colostral and milk mononuclear cells expressed a different cytokine transcript expression pattern regarding the vaccine used. These results demonstrated that the colostral cellular population was active and could exert its action influencing the final immune response.

AB - In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing neutralizing antibodies (NAb), suggesting that another immunological effectors were involved in the protection observed. In this work, groups of cows (n= 4) were immunized in the last third of gestation with a bovine rotavirus (BRV) experimental vaccine and with a VP6 subunit vaccine. At birth, colostrums from vaccinated and non-vaccinated cows were processed and viable colostral mononuclear cells were obtained. With the purpose of determining the cytokine patterns generated by cells from immune secretions (colostrums and milk), a relative quantification by real time PCR was standardized. Quantitative real time PCR (qPCR) was used to determine transcript levels of IL-4, IL-6, IL-10, IL-12, IFN-γ and IFN-α from these cells. Colostral and milk mononuclear cells expressed a different cytokine transcript expression pattern regarding the vaccine used. These results demonstrated that the colostral cellular population was active and could exert its action influencing the final immune response.

KW - Colostrum cells

KW - QPCR relative method

KW - VP6 subunit vaccine

UR - http://www.scopus.com/inward/record.url?scp=84881558856&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881558856&partnerID=8YFLogxK

U2 - 10.1016/j.rvsc.2013.03.016

DO - 10.1016/j.rvsc.2013.03.016

M3 - Article

C2 - 23602433

AN - SCOPUS:84881558856

VL - 95

SP - 703

EP - 708

JO - Research in Veterinary Science

JF - Research in Veterinary Science

SN - 0034-5288

IS - 2

ER -