Abstract
In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing neutralizing antibodies (NAb), suggesting that another immunological effectors were involved in the protection observed. In this work, groups of cows (n= 4) were immunized in the last third of gestation with a bovine rotavirus (BRV) experimental vaccine and with a VP6 subunit vaccine. At birth, colostrums from vaccinated and non-vaccinated cows were processed and viable colostral mononuclear cells were obtained. With the purpose of determining the cytokine patterns generated by cells from immune secretions (colostrums and milk), a relative quantification by real time PCR was standardized. Quantitative real time PCR (qPCR) was used to determine transcript levels of IL-4, IL-6, IL-10, IL-12, IFN-γ and IFN-α from these cells. Colostral and milk mononuclear cells expressed a different cytokine transcript expression pattern regarding the vaccine used. These results demonstrated that the colostral cellular population was active and could exert its action influencing the final immune response.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 703-708 |
| Number of pages | 6 |
| Journal | Research in veterinary science |
| Volume | 95 |
| Issue number | 2 |
| DOIs | |
| State | Published - Oct 1 2013 |
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Keywords
- Colostrum cells
- QPCR relative method
- VP6 subunit vaccine
Cite this
Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with a bovine rotavirus VP6 experimental vaccine. / Gonzalez, D. D.; Rimondi, A.; Perez Aguirreburualde, M. S.; Mozgovoj, M.; Bellido, D.; Wigdorovitz, A.; Dus Santos, M. J.
In: Research in veterinary science, Vol. 95, No. 2, 01.10.2013, p. 703-708.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with a bovine rotavirus VP6 experimental vaccine
AU - Gonzalez, D. D.
AU - Rimondi, A.
AU - Perez Aguirreburualde, M. S.
AU - Mozgovoj, M.
AU - Bellido, D.
AU - Wigdorovitz, A.
AU - Dus Santos, M. J.
PY - 2013/10/1
Y1 - 2013/10/1
N2 - In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing neutralizing antibodies (NAb), suggesting that another immunological effectors were involved in the protection observed. In this work, groups of cows (n= 4) were immunized in the last third of gestation with a bovine rotavirus (BRV) experimental vaccine and with a VP6 subunit vaccine. At birth, colostrums from vaccinated and non-vaccinated cows were processed and viable colostral mononuclear cells were obtained. With the purpose of determining the cytokine patterns generated by cells from immune secretions (colostrums and milk), a relative quantification by real time PCR was standardized. Quantitative real time PCR (qPCR) was used to determine transcript levels of IL-4, IL-6, IL-10, IL-12, IFN-γ and IFN-α from these cells. Colostral and milk mononuclear cells expressed a different cytokine transcript expression pattern regarding the vaccine used. These results demonstrated that the colostral cellular population was active and could exert its action influencing the final immune response.
AB - In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing neutralizing antibodies (NAb), suggesting that another immunological effectors were involved in the protection observed. In this work, groups of cows (n= 4) were immunized in the last third of gestation with a bovine rotavirus (BRV) experimental vaccine and with a VP6 subunit vaccine. At birth, colostrums from vaccinated and non-vaccinated cows were processed and viable colostral mononuclear cells were obtained. With the purpose of determining the cytokine patterns generated by cells from immune secretions (colostrums and milk), a relative quantification by real time PCR was standardized. Quantitative real time PCR (qPCR) was used to determine transcript levels of IL-4, IL-6, IL-10, IL-12, IFN-γ and IFN-α from these cells. Colostral and milk mononuclear cells expressed a different cytokine transcript expression pattern regarding the vaccine used. These results demonstrated that the colostral cellular population was active and could exert its action influencing the final immune response.
KW - Colostrum cells
KW - QPCR relative method
KW - VP6 subunit vaccine
UR - http://www.scopus.com/inward/record.url?scp=84881558856&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84881558856&partnerID=8YFLogxK
U2 - 10.1016/j.rvsc.2013.03.016
DO - 10.1016/j.rvsc.2013.03.016
M3 - Article
C2 - 23602433
AN - SCOPUS:84881558856
VL - 95
SP - 703
EP - 708
JO - Research in Veterinary Science
JF - Research in Veterinary Science
SN - 0034-5288
IS - 2
ER -