Quantitation of antral gastrin cell populations in the dog

John P. Delaney, Hector Mejia Michel, M. Michael Eisenberg, Margaret Bonsack

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

A method for enumerating G cells in dog antral mucosa by a fluorescent antibody technique was shown to be valid with respect to specificity and reproducibility. The state of intracellular gastrin storage does not influence G cell identification by this technique. To obtain values representative of the entire antrum, all G cells in long strips of antral mucosa must be counted. Results are best expressed as G cells per centimeter length of mucosa. Even with large mucosal samples there are considerable variations in G cell density within a single antrum. The "average" dog antrum contains about 35 million G cells. We observed as much as 2-fold range in G cell density from one dog to another. Each animal should, therefore, serve as its own control when influences on G cell populations are to be studied experimentally.

Original languageEnglish (US)
Pages (from-to)708-712
Number of pages5
JournalGastroenterology
Volume74
Issue number4
DOIs
StatePublished - Apr 1978

Fingerprint Dive into the research topics of 'Quantitation of antral gastrin cell populations in the dog'. Together they form a unique fingerprint.

Cite this