Abstract
Proteins are involved in nearly every biological process, which makes them of interest to a range of scientists. Previous work has shown that hand-held cameras can be used to determine the concentration of colored analytes in solution, and this paper extends the approach to reactions involving a color change in order to quantify protein concentration (e.g., green to blue). Herein, we describe the successful use of smartphone colorimetry to quantify protein concentration using two common colorimetric biochemical methods, the Bradford and biuret assays. The ease of the experimental setup makes these lab experiments accessible to a wide range of students and can be used as both high school and college level laboratory experiments.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 941-945 |
| Number of pages | 5 |
| Journal | Journal of Chemical Education |
| Volume | 94 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 11 2017 |
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Keywords
- Analytical Chemistry
- Biochemistry
- First-Year Undergraduate/General
- Hands-On Learning/Manipulatives
- High School/Introductory Chemistry
- Laboratory Instruction
- Proteins/Peptides
- Quantitative Analysis
- UV-Vis Spectroscopy
Cite this
Quantifying Protein Concentrations Using Smartphone Colorimetry : A New Method for an Established Test. / Gee, Clifford T.; Kehoe, Eric; Pomerantz, William C; Penn, Lee.
In: Journal of Chemical Education, Vol. 94, No. 7, 11.07.2017, p. 941-945.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantifying Protein Concentrations Using Smartphone Colorimetry
T2 - A New Method for an Established Test
AU - Gee, Clifford T.
AU - Kehoe, Eric
AU - Pomerantz, William C
AU - Penn, Lee
PY - 2017/7/11
Y1 - 2017/7/11
N2 - Proteins are involved in nearly every biological process, which makes them of interest to a range of scientists. Previous work has shown that hand-held cameras can be used to determine the concentration of colored analytes in solution, and this paper extends the approach to reactions involving a color change in order to quantify protein concentration (e.g., green to blue). Herein, we describe the successful use of smartphone colorimetry to quantify protein concentration using two common colorimetric biochemical methods, the Bradford and biuret assays. The ease of the experimental setup makes these lab experiments accessible to a wide range of students and can be used as both high school and college level laboratory experiments.
AB - Proteins are involved in nearly every biological process, which makes them of interest to a range of scientists. Previous work has shown that hand-held cameras can be used to determine the concentration of colored analytes in solution, and this paper extends the approach to reactions involving a color change in order to quantify protein concentration (e.g., green to blue). Herein, we describe the successful use of smartphone colorimetry to quantify protein concentration using two common colorimetric biochemical methods, the Bradford and biuret assays. The ease of the experimental setup makes these lab experiments accessible to a wide range of students and can be used as both high school and college level laboratory experiments.
KW - Analytical Chemistry
KW - Biochemistry
KW - First-Year Undergraduate/General
KW - Hands-On Learning/Manipulatives
KW - High School/Introductory Chemistry
KW - Laboratory Instruction
KW - Proteins/Peptides
KW - Quantitative Analysis
KW - UV-Vis Spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=85022325662&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85022325662&partnerID=8YFLogxK
U2 - 10.1021/acs.jchemed.6b00676
DO - 10.1021/acs.jchemed.6b00676
M3 - Article
AN - SCOPUS:85022325662
VL - 94
SP - 941
EP - 945
JO - Journal of Chemical Education
JF - Journal of Chemical Education
SN - 0021-9584
IS - 7
ER -