TY - JOUR
T1 - Quantification of antibiotic resistance genes for environmental monitoring
T2 - Current methods and future directions
AU - Ishii, Satoshi
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/8
Y1 - 2020/8
N2 - Various antibiotic resistance genes (ARGs) could be present in environments such as soil and water. This review and opinion article briefly summarizes on the current methods to quantify ARGs in these environments, with special emphasis on their application potentials for routine ARG monitoring. For routine ARG monitoring, methods should be rapid, specific, sensitive, and easy-to-use. Quantitative data are also important if removal efficiencies of ARGs during treatment processes or potential human health risks are evaluated/assessed. To meet these requirements, culture-independent, amplification-based methods are most promising, including quantitative polymerase chain reaction, high-throughput quantitative polymerase chain reaction, digital PCR, and loop-mediated isothermal amplification. Selection of target genes is also important for routine ARG monitoring. Future research may include the identification of ARG indicators that can predict the occurrence and the levels of various ARGs.
AB - Various antibiotic resistance genes (ARGs) could be present in environments such as soil and water. This review and opinion article briefly summarizes on the current methods to quantify ARGs in these environments, with special emphasis on their application potentials for routine ARG monitoring. For routine ARG monitoring, methods should be rapid, specific, sensitive, and easy-to-use. Quantitative data are also important if removal efficiencies of ARGs during treatment processes or potential human health risks are evaluated/assessed. To meet these requirements, culture-independent, amplification-based methods are most promising, including quantitative polymerase chain reaction, high-throughput quantitative polymerase chain reaction, digital PCR, and loop-mediated isothermal amplification. Selection of target genes is also important for routine ARG monitoring. Future research may include the identification of ARG indicators that can predict the occurrence and the levels of various ARGs.
KW - Antibiotic resistance genes
KW - Digital PCR
KW - Isothermal amplification
KW - Quantitative PCR
KW - Routine monitoring
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U2 - 10.1016/j.coesh.2020.02.004
DO - 10.1016/j.coesh.2020.02.004
M3 - Review article
AN - SCOPUS:85082799557
SN - 2468-5844
VL - 16
SP - 47
EP - 53
JO - Current Opinion in Environmental Science and Health
JF - Current Opinion in Environmental Science and Health
ER -