QTL mapping of net blotch resistance genes in a doubled-haploid population of six-rowed barley

Zhengqiang Ma, Nora L.V. Lapitan, Brian Steffenson

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


Net blotch, caused by Pyrenophora teres f. teres, is a damaging foliar disease of barley worldwide. It is important to identify resistance germplasm and study their genetics. 'Chevron', a six-rowed barley used as a parent for the production of a doubled haploid (DH) population for mapping of Fusarium head blight (FHB) resistance, was also found to be resistant to net blotch. To map the resistance genes, the population was evaluated for resistance at the seedling stage in a greenhouse. The resistance data showed a two-peak distribution. Through linkage mapping, one resistance gene, tentatively called Rpt, was located on chromosome 6HS flanked by Xksua3b-Xwg719d, which was also detected by QTL mapping. This QTL explained 64% of the phenotypic variance for the resistance in this DH population. In addition, a minor QTL was found on chromosome 2HS defined by Xcdo786-Xabc156a. 'Chevron' and 'Stander' contributed the resistant alleles of Rpt and the 2HS QTL, respectively. Both QTLs together explained nearly 70% of the phenotypic variance. The markers for these QTLs are useful for marker-assisted selection of net blotch resistance in barley breeding.

Original languageEnglish (US)
Pages (from-to)291-296
Number of pages6
Issue number3
StatePublished - Jun 2004

Bibliographical note

Funding Information:
This project was partially supported by the North American Barley Mapping Project (NABGMP), American Malting Barley Association, Anheuser Busch, and Hatch Funds 644. The fund to the first author was partially supported by the Outstanding Youth Fund (No. 30025030) of the National Science Foundation of China and ‘973’ project (No. G1998010200) and Fund for Key Faculties in Higher Education of China.


  • QTL
  • RFLP
  • barley
  • mapping
  • net blotch
  • resistance


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