Purification of skeletal-muscle mitochondria by density-gradient centrifugation with Percoll

James R. Mickelson; Marion L. Greaser; B. Bruce Marsh

doi:10.1016/0003-2697(80)90645-4

Purification of skeletal-muscle mitochondria by density-gradient centrifugation with Percoll

James R. Mickelson, Marion L. Greaser, B. Bruce Marsh

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Mitochondria prepared from skeletal muscle are typically contaminated with sarcoplasmic reticulum fragments and salt-soluble proteins. These contaminants can be removed by density-gradient centrifugation in Percoll. The resulting mitochondria retain both their original state three respiratory rates and their respiratory control ratios. The method has also been adapted to prepare mitochondria from very small tissue samples.

Original language	English (US)
Pages (from-to)	255-260
Number of pages	6
Journal	Analytical Biochemistry
Volume	109
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(80)90645-4
State	Published - Dec 1980

Bibliographical note

Funding Information:
’ This research was supported by the College of Agricuhural and Life Sciences, University of Wisconsin, Madison. Wisconsin. Muscle Biology Manuscript No. 146.

Publisher link

10.1016/0003-2697(80)90645-4

Find It

Find It at U of M Libraries

Cite this

@article{6650f1bbee174baeb0946acef06f2ad6,

title = "Purification of skeletal-muscle mitochondria by density-gradient centrifugation with Percoll",

abstract = "Mitochondria prepared from skeletal muscle are typically contaminated with sarcoplasmic reticulum fragments and salt-soluble proteins. These contaminants can be removed by density-gradient centrifugation in Percoll. The resulting mitochondria retain both their original state three respiratory rates and their respiratory control ratios. The method has also been adapted to prepare mitochondria from very small tissue samples.",

author = "Mickelson, {James R.} and Greaser, {Marion L.} and Marsh, {B. Bruce}",

note = "Funding Information: {\textquoteright} This research was supported by the College of Agricuhural and Life Sciences, University of Wisconsin, Madison. Wisconsin. Muscle Biology Manuscript No. 146.",

year = "1980",

month = dec,

doi = "10.1016/0003-2697(80)90645-4",

language = "English (US)",

volume = "109",

pages = "255--260",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Purification of skeletal-muscle mitochondria by density-gradient centrifugation with Percoll

AU - Mickelson, James R.

AU - Greaser, Marion L.

AU - Marsh, B. Bruce

N1 - Funding Information: ’ This research was supported by the College of Agricuhural and Life Sciences, University of Wisconsin, Madison. Wisconsin. Muscle Biology Manuscript No. 146.

PY - 1980/12

Y1 - 1980/12

N2 - Mitochondria prepared from skeletal muscle are typically contaminated with sarcoplasmic reticulum fragments and salt-soluble proteins. These contaminants can be removed by density-gradient centrifugation in Percoll. The resulting mitochondria retain both their original state three respiratory rates and their respiratory control ratios. The method has also been adapted to prepare mitochondria from very small tissue samples.

AB - Mitochondria prepared from skeletal muscle are typically contaminated with sarcoplasmic reticulum fragments and salt-soluble proteins. These contaminants can be removed by density-gradient centrifugation in Percoll. The resulting mitochondria retain both their original state three respiratory rates and their respiratory control ratios. The method has also been adapted to prepare mitochondria from very small tissue samples.

UR - http://www.scopus.com/inward/record.url?scp=0019268144&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019268144&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(80)90645-4

DO - 10.1016/0003-2697(80)90645-4

M3 - Article

C2 - 6261608

AN - SCOPUS:0019268144

SN - 0003-2697

VL - 109

SP - 255

EP - 260

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Purification of skeletal-muscle mitochondria by density-gradient centrifugation with Percoll

Abstract

Bibliographical note

Publisher link

Find It

Other files and links

Fingerprint

Cite this