Purification and crystallization of fumarase C from Escherichia coli

Todd M. Weaver, David G Levitt, Leonard J. Banaszak

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Fumarase C purified from Escherichia coli has been crystallized in the presence of polyethylene glycol in both a citrate buffer at pH 5.3 and a 3-(4-morpholino)-propanesulfonic acid buffer at pH 7.5 yielding two crystal forms. An orthorhombic C2221 form was obtained in citrate at pH 5.3 and an orthorhombic I222 form was obtained in 3-(4-morpholino)-propanesulfonic acid (pH 7.5). Complete native data sets have been collected on both crystal forms: the C2221 form is complete to 2.10 Å and the I222 form is complete to 2.20 Å.

Original languageEnglish (US)
Pages (from-to)141-144
Number of pages4
JournalJournal of Molecular Biology
Volume231
Issue number1
DOIs
StatePublished - May 1993

Keywords

  • Citric acid cycle
  • Fum C
  • Fumarase
  • Fumarase C
  • Sox RS

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