TY - JOUR
T1 - Purification and characterization of a glucosamine-binding antifungal lectin from phaseolus vulgaris cv. Chinese Pinto Beans with antiproliferative activity towards nasopharyngeal carcinoma cells
AU - Ang, Andrew Si Wo
AU - Cheung, Randy Chi Fai
AU - Dan, Xiuli
AU - Chan, Yau Sang
AU - Pan, Wenliang
AU - Ng, Tzi Bun
PY - 2014/1
Y1 - 2014/1
N2 - A lectin has successfully been isolated from Phaseolus vulgaris cv. Chinese pinto bean using affinity chromatography, ion exchange chromatography, and gel filtration in succession, with a 15.4-fold purification. Investigation of its characteristics revealed that Chinese pinto bean lectin (CPBL) was a 58-kDa dimeric glucosamine-binding protein. Its Mg2+-dependent hemagglutinating activity was stable at pH 7-8 and at or below 60 C. When the purified lectin was tested against six fungal species including Phyllosticta citriasiana, Magnaporthe grisea, Bipolans maydis, Valsa mali, Mycosphaerella arachidicola, and Setosphaeria turcica, only the mycelial growth of V. mali was reduced by 30.6 % by the lectin at 30 μM. The lectin did not exert any discernible antiproliferative effects on breast cancer MCF-7 cells, but was able to suppress proliferation of nasopharyngeal carcinoma HONE-1 cells, with an IC50 of 17.3 μM, as revealed by the MTT assay. Since few plant lectins demonstrate antifungal activity against V. mali, and not many others have inhibitory effects on HONE-1 cells, CPBL is a distinctive lectin which may be exploited for development into an agent against V. mali and HONE-1 cells.
AB - A lectin has successfully been isolated from Phaseolus vulgaris cv. Chinese pinto bean using affinity chromatography, ion exchange chromatography, and gel filtration in succession, with a 15.4-fold purification. Investigation of its characteristics revealed that Chinese pinto bean lectin (CPBL) was a 58-kDa dimeric glucosamine-binding protein. Its Mg2+-dependent hemagglutinating activity was stable at pH 7-8 and at or below 60 C. When the purified lectin was tested against six fungal species including Phyllosticta citriasiana, Magnaporthe grisea, Bipolans maydis, Valsa mali, Mycosphaerella arachidicola, and Setosphaeria turcica, only the mycelial growth of V. mali was reduced by 30.6 % by the lectin at 30 μM. The lectin did not exert any discernible antiproliferative effects on breast cancer MCF-7 cells, but was able to suppress proliferation of nasopharyngeal carcinoma HONE-1 cells, with an IC50 of 17.3 μM, as revealed by the MTT assay. Since few plant lectins demonstrate antifungal activity against V. mali, and not many others have inhibitory effects on HONE-1 cells, CPBL is a distinctive lectin which may be exploited for development into an agent against V. mali and HONE-1 cells.
KW - Antifungal
KW - Antitumor
KW - Lectin
KW - Nasopharyngeal carcinomas
KW - Phaseolus vulgaris
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UR - http://www.scopus.com/inward/citedby.url?scp=84894505227&partnerID=8YFLogxK
U2 - 10.1007/s12010-013-0542-2
DO - 10.1007/s12010-013-0542-2
M3 - Article
C2 - 24114321
AN - SCOPUS:84894505227
SN - 0273-2289
VL - 172
SP - 672
EP - 686
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 2
ER -