Prunus yedoensis inhibits the inflammatory chemokines, MDC and TARC, by regulating the STAT1-signaling pathway in IFN-γ-stimulated HaCaT human keratinocytes

Gyeoung Jin Kang, Hye Ja Lee, Weon Jong Yoon, Eun Jin Yang, Sun Son Park, Hee Kyoung Kang, Myung Hwan Park, Eun Sook Yoo

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Atopic dermatitis (AD) is an inflammatory skin disease commonly characterized by infiltration of inflammatory cells into skin lesions. Keratinocytes produce many chemokines that are involved in the pathogenesis of skin disorders. In particular, macrophage-derived chemokine (MDC/CCL22) and thymus and activation-regulated chemokine (TARC/CCL17) are Th2-type cytokines. Serum MDC and TARC levels are increased in AD patients. In this study, we investigated the anti-inflammatory effect and mechanism of action of the active fraction from Prunus yedoensis bark. We evaluated their inhibitory effects on the AD-like inflammatory markers (MDC and TARC) and JAK-STAT pathway (STAT1) in HaCaT keratinocytes. The EtOAc fraction of the crude extract (80% EtOH) and the E5 sub-fraction potently inhibited the induction of MDC and TARC mRNA and protein at 50 μg/mL in HaCaT cells. In addition, the E5 sub-fraction inhibited the phosphorylation of STAT1 protein associated with IFN-γ signaling transduction in a dose-dependent manner. Thus, P. yedoensis may have antiatopic activity by suppressing the inflammatory chemokines (MDC and TARC).

Original languageEnglish (US)
Pages (from-to)394-402
Number of pages9
JournalBiomolecules and Therapeutics
Volume16
Issue number4
DOIs
StatePublished - Dec 2008

Keywords

  • Atopic dermatitis (AD)
  • HaCaT keratinocytes
  • Jak-STAT pathway
  • MDC (CCL22)
  • Prunus yedoensis
  • TARC (CCL17)

Fingerprint Dive into the research topics of 'Prunus yedoensis inhibits the inflammatory chemokines, MDC and TARC, by regulating the STAT1-signaling pathway in IFN-γ-stimulated HaCaT human keratinocytes'. Together they form a unique fingerprint.

Cite this