Protoplast transformation of filamentous fungi.

B. Gillian Turgeon; Bradford Condon; Jinyuan Liu; Ning Zhang

doi:10.1007/978-1-60761-611-5_1

Protoplast transformation of filamentous fungi.

B. Gillian Turgeon
, Bradford Condon
, Jinyuan Liu
, Ning Zhang

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed.

Original language	English (US)
Pages (from-to)	3-19
Number of pages	17
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	638
DOIs	https://doi.org/10.1007/978-1-60761-611-5_1
State	Published - 2010
Externally published	Yes

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title = "Protoplast transformation of filamentous fungi.",

abstract = "The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed.",

author = "Turgeon, \{B. Gillian\} and Bradford Condon and Jinyuan Liu and Ning Zhang",

year = "2010",

doi = "10.1007/978-1-60761-611-5\_1",

language = "English (US)",

volume = "638",

pages = "3--19",

journal = "Methods in molecular biology (Clifton, N.J.)",

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TY - JOUR

T1 - Protoplast transformation of filamentous fungi.

AU - Turgeon, B. Gillian

AU - Condon, Bradford

AU - Liu, Jinyuan

AU - Zhang, Ning

PY - 2010

Y1 - 2010

N2 - The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed.

AB - The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed.

UR - https://www.scopus.com/pages/publications/77953632873

UR - https://www.scopus.com/pages/publications/77953632873#tab=citedBy

U2 - 10.1007/978-1-60761-611-5_1

DO - 10.1007/978-1-60761-611-5_1

M3 - Article

C2 - 20238257

AN - SCOPUS:77953632873

SN - 1064-3745

VL - 638

SP - 3

EP - 19

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Protoplast transformation of filamentous fungi.

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