Protoplast transformation of filamentous fungi.

B. Gillian Turgeon, Bradford Condon, Jinyuan Liu, Ning Zhang

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed.

Original languageEnglish (US)
Pages (from-to)3-19
Number of pages17
JournalMethods in molecular biology (Clifton, N.J.)
Volume638
DOIs
StatePublished - 2010
Externally publishedYes

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