Protocol to establish turkey oviductal organoids as an in vitro model

Pitchaya Santativongchai, Caitlin C. Klaeui, Sunantha Kosonsiriluk, Milena Saqui-Salces, Kent M. Reed, Ben W. Wileman, Marissa M. Studniski, Kahina S. Boukherroub

Research output: Contribution to journalArticlepeer-review

Abstract

The study of reproductive function in turkey hens has been difficult due to the lack of a reliable, representative in vitro model for investigating profound physiological aspects. This article presents a protocol to establish turkey oviductal organoids, including steps for isolating turkey oviduct epithelial cells followed by seeding and maintaining 3D organoid cultures. We also detail procedures for organoid fixation for histological analysis. This organoid model could serve as a valuable in vitro tool for understanding the intricacies of turkey reproductive physiology.

Original languageEnglish (US)
Article number103384
JournalSTAR Protocols
Volume5
Issue number4
DOIs
StatePublished - Dec 20 2024

Bibliographical note

Publisher Copyright:
© 2024 The Author(s)

Keywords

  • cell biology
  • organoids
  • tissue engineering

PubMed: MeSH publication types

  • Journal Article

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