Abstract
The study of reproductive function in turkey hens has been difficult due to the lack of a reliable, representative in vitro model for investigating profound physiological aspects. This article presents a protocol to establish turkey oviductal organoids, including steps for isolating turkey oviduct epithelial cells followed by seeding and maintaining 3D organoid cultures. We also detail procedures for organoid fixation for histological analysis. This organoid model could serve as a valuable in vitro tool for understanding the intricacies of turkey reproductive physiology.
Original language | English (US) |
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Article number | 103384 |
Journal | STAR Protocols |
Volume | 5 |
Issue number | 4 |
DOIs | |
State | Published - Dec 20 2024 |
Bibliographical note
Publisher Copyright:© 2024 The Author(s)
Keywords
- cell biology
- organoids
- tissue engineering
PubMed: MeSH publication types
- Journal Article