Protocol for applying Tumor Treating Fields in mouse models of cancer using the inovivo system

Travis J. Gates; Sewar Zbidat; Karina Deniz; Sanyukta Padmanabhan; Katherine Ladner; Akshat Sarkari; Xianda Zhao; Shiri Davidi; Roni Blatt; Shay Cahal; Martin Gabay; Mariell Sellevoll; Itai Tzchori; Yaara Porat; Adi Haber; Moshe Giladi; Subbaya Subramanian; Emil Lou

doi:10.1016/j.xpro.2024.103535

Protocol for applying Tumor Treating Fields in mouse models of cancer using the inovivo system

Travis J. Gates
, Sewar Zbidat
, Karina Deniz
, Sanyukta Padmanabhan
, Katherine Ladner
, Akshat Sarkari
, Xianda Zhao
, Shiri Davidi
, Roni Blatt
, Shay Cahal
, Martin Gabay
, Mariell Sellevoll
, Itai Tzchori
, Yaara Porat
, Adi Haber
, Moshe Giladi
, Subbaya Subramanian
, Emil Lou

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Tumor Treating Fields (TTFields) are electric fields clinically approved for cancer treatment, delivered via arrays attached to the patient's skin. Here, we present a protocol for applying TTFields to torso orthotopic and subcutaneous mouse tumor models using the inovivo system. We guide users on proper system component connections, study protocol design, mouse fur depilation, array application, and treatment condition adjustment and monitoring. The inovivo system allows for the concurrent application of TTFields with standard cancer therapies. For complete details on the use and execution of this protocol, please refer to Barsheshet et al.¹

Original language	English (US)
Article number	103535
Journal	STAR Protocols
Volume	6
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2024.103535
State	Published - Mar 21 2025

Bibliographical note

Publisher Copyright:
© 2024 The Authors

Keywords

cancer
cell biology
model organisms

PubMed: MeSH publication types

Journal Article

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.xpro.2024.103535

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Gates, T. J., Zbidat, S., Deniz, K., Padmanabhan, S., Ladner, K., Sarkari, A., Zhao, X., Davidi, S., Blatt, R., Cahal, S., Gabay, M., Sellevoll, M., Tzchori, I., Porat, Y., Haber, A., Giladi, M., Subramanian, S., & Lou, E. (2025). Protocol for applying Tumor Treating Fields in mouse models of cancer using the inovivo system. STAR Protocols, 6(1), Article 103535. https://doi.org/10.1016/j.xpro.2024.103535

Gates, TJ, Zbidat, S, Deniz, K, Padmanabhan, S, Ladner, K, Sarkari, A, Zhao, X, Davidi, S, Blatt, R, Cahal, S, Gabay, M, Sellevoll, M, Tzchori, I, Porat, Y, Haber, A, Giladi, M, Subramanian, S & Lou, E 2025, 'Protocol for applying Tumor Treating Fields in mouse models of cancer using the inovivo system', STAR Protocols, vol. 6, no. 1, 103535. https://doi.org/10.1016/j.xpro.2024.103535

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title = "Protocol for applying Tumor Treating Fields in mouse models of cancer using the inovivo system",

abstract = "Tumor Treating Fields (TTFields) are electric fields clinically approved for cancer treatment, delivered via arrays attached to the patient's skin. Here, we present a protocol for applying TTFields to torso orthotopic and subcutaneous mouse tumor models using the inovivo system. We guide users on proper system component connections, study protocol design, mouse fur depilation, array application, and treatment condition adjustment and monitoring. The inovivo system allows for the concurrent application of TTFields with standard cancer therapies. For complete details on the use and execution of this protocol, please refer to Barsheshet et al.1",

keywords = "cancer, cell biology, model organisms",

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doi = "10.1016/j.xpro.2024.103535",

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AU - Gates, Travis J.

AU - Zbidat, Sewar

AU - Deniz, Karina

AU - Padmanabhan, Sanyukta

AU - Ladner, Katherine

AU - Sarkari, Akshat

AU - Zhao, Xianda

AU - Davidi, Shiri

AU - Blatt, Roni

AU - Cahal, Shay

AU - Gabay, Martin

AU - Sellevoll, Mariell

AU - Tzchori, Itai

AU - Porat, Yaara

AU - Haber, Adi

AU - Giladi, Moshe

AU - Subramanian, Subbaya

AU - Lou, Emil

PY - 2025/3/21

Y1 - 2025/3/21

N2 - Tumor Treating Fields (TTFields) are electric fields clinically approved for cancer treatment, delivered via arrays attached to the patient's skin. Here, we present a protocol for applying TTFields to torso orthotopic and subcutaneous mouse tumor models using the inovivo system. We guide users on proper system component connections, study protocol design, mouse fur depilation, array application, and treatment condition adjustment and monitoring. The inovivo system allows for the concurrent application of TTFields with standard cancer therapies. For complete details on the use and execution of this protocol, please refer to Barsheshet et al.1

AB - Tumor Treating Fields (TTFields) are electric fields clinically approved for cancer treatment, delivered via arrays attached to the patient's skin. Here, we present a protocol for applying TTFields to torso orthotopic and subcutaneous mouse tumor models using the inovivo system. We guide users on proper system component connections, study protocol design, mouse fur depilation, array application, and treatment condition adjustment and monitoring. The inovivo system allows for the concurrent application of TTFields with standard cancer therapies. For complete details on the use and execution of this protocol, please refer to Barsheshet et al.1

KW - cancer

KW - cell biology

KW - model organisms

UR - https://www.scopus.com/pages/publications/85214328732

UR - https://www.scopus.com/pages/publications/85214328732#tab=citedBy

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DO - 10.1016/j.xpro.2024.103535

M3 - Article

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SN - 2666-1667

VL - 6

JO - STAR Protocols

JF - STAR Protocols

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M1 - 103535

ER -

Protocol for applying Tumor Treating Fields in mouse models of cancer using the inovivo system

Abstract

Bibliographical note

Keywords

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UN SDGs

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