Proteomics for the diagnosis of thyroid lesions: Preliminary report

F. Pagni, V. Mainini, M. Garancini, F. Bono, A. Vanzati, V. Giardini, M. Scardilli, P. Goffredo, A. J. Smith, M. Galli, G. De Sio, F. Magni

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


Objective: Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a unique proteomic technology that explores the spatial distribution of biomolecules directly in situ, thus integrating molecular and morphological information. The possibility of correlating distribution maps of multiple analyses with cytological features makes it an ideal research tool for discovering new diagnostic markers. A previous study showed that MALDI-IMS could help discrimination between different types of thyroid lesions, especially papillary thyroid carcinoma (PTC); the present feasibility study on ex vivo fine needle aspiration (FNA) smears describes its potential in detecting new proteomic targets of other thyroid lesions (follicular lesions, medullary carcinoma). Methods: MALDI-IMS was conducted on ex vivo FNAs obtained from surgical specimens and corresponding in vivo samples. Differences between proteomic profiles of different thyroid lesions were compared. Results: Comparing the protein profiles of hyperplastic nodules obtained from three different patients with each other, and with a new PTC, showed a high degree of concordance, indicating good reproducibility of the IMS technology on cytological samples, suggesting its potential as a tool for biomarker discovery. Furthermore, comparison of the average proteomic profiles of hyperplastic nodules with a Hürthle cell adenoma revealed significant differences, underlying the capability of MALDI-IMS to distinguish between different thyroid lesions. Finally, the proteomic profile of medullary thyroid carcinoma was also characterized. Conclusions: Our results confirmed the possible role of MALDI-IMS in the search for diagnostic targets of PTC and follicular lesions, which could be applied in larger trials aimed at the identification of proteins, convertible to cost-effective diagnostic tools such as immunohistochemistry. These tests could be used to analyse in vivo cytological smears, improving the preoperative diagnosis of indeterminate thyroid nodules.

Original languageEnglish (US)
Pages (from-to)318-324
Number of pages7
Issue number5
StatePublished - Oct 1 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2015 John Wiley & Sons Ltd.


  • Fine needle aspiration cytology
  • Imaging mass spectrometry
  • MALDI-IMS; thyroid follicular lesions
  • Matrix-assisted laser desorption/ionization
  • Thyroid carcinoma; proteomics


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