Proteomic profiling of the epileptic dentate gyrus

Aiqing Li, Yun Sik Choi, Heather Dziema, Ruifeng Cao, Hee Yeon Cho, Yeon Joo Jung, Karl Obrietan

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The development of epilepsy is often associated with marked changes in central nervous system cell structure and function. Along these lines, reactive gliosis and granule cell axonal sprouting within the dentate gyrus of the hippocampus are commonly observed in individuals with temporal lobe epilepsy (TLE). Here we used the pilocarpine model of TLE in mice to screen the proteome and phosphoproteome of the dentate gyrus to identify molecular events that are altered as part of the pathogenic process. Using a two-dimensional gel electrophoresis-based approach, followed by liquid chromatography-tandem mass spectrometry, 24 differentially expressed proteins, including 9 phosphoproteins, were identified. Functionally, these proteins were organized into several classes, including synaptic physiology, cell structure, cell stress, metabolism and energetics. The altered expression of three proteins involved in synaptic physiology, actin, profilin 1 and α-synuclein was validated by secondary methods. Interestingly, marked changes in protein expression were detected in the supragranular cell region, an area where robust mossy fibers sprouting occurs. Together, these data provide new molecular insights into the altered protein profile of the epileptogenic dentate gyrus and point to potential pathophysiologic mechanisms underlying epileptogenesis.

Original languageEnglish (US)
Pages (from-to)1077-1089
Number of pages13
JournalBrain Pathology
Volume20
Issue number6
DOIs
StatePublished - Nov 2010

Keywords

  • dentate gyrus
  • epilepsy
  • granule cell
  • pilocarpine
  • status epilepticus

Fingerprint Dive into the research topics of 'Proteomic profiling of the epileptic dentate gyrus'. Together they form a unique fingerprint.

Cite this